Proteomics

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Brittonodoxa subpinnata soluble extract


ABSTRACT: For HPLC-MS2 analysis, 50 mg of plant material was extracted with 5 mL of 80% methanol. The supernatant was collected and submitted to a cleanup process through a C18 SPE by activating the column with methanol and equilibrating it with ultrapure water. The extract was loaded into the column and eluted with 5 mL of the following solvents: ultrapure water, followed by 25% methanol, 50% methanol, and washed with methanol. The fractions were joined, transferred to a tube, and vacuum dried.

INSTRUMENT(S): HPLC-MS ESI low resolution (Thermo-Fischer)

ORGANISM(S): Brittonodoxa Subpinnata

SUBMITTER: Wilton Ricardo Sala-Carvalho  

PROVIDER: MSV000093497 | MassIVE | Sat Nov 25 14:04:00 GMT 2023

REPOSITORIES: MassIVE

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