Native metabolomics with E.coli CutA and Isosepiapterin
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ABSTRACT: Native MS with E.coli CutA and Isosepiapterin to check for putative binding. Isosepiapterin was dissolved in 50% MeOH and run over a C18 column.
Project description:Native MS with E.coli CutA and Isosepiapterin to check for putative binding. Isosepiapterin was dissolved in 50% MeOH and run over a C18 column.
Project description:Native and non-targeted metabolomics with E.coli CutA and cell extracts of E.coli and Synechococcus elongatus PCC 7942. Cell extraction with 20% or 80% MeOH.
Project description:Native and non-targeted metabolomics with E.coli CutA and cell extracts of E.coli and Synechococcus elongatus PCC 7942. Cell extraction with 20% or 80% MeOH.
Project description:E.coli CutA was flow injected against cell extracts (cell pellets - of exponential growing cultures- have been extracted with 20% or 80% MeOH) of E.coli BW24113 and Synechococcus elongatus sp. PCC 7942 WT or deltacutA
Project description:Native metabolomics was performed using CutA proteins of Synechococcus elongatus sp. PCC 7942 or E.coli. Crude cell extract or a mix of co-purified polar compounds were offered as putative binding partners.
Project description:Native metabolomics was performed using CutA proteins of Synechococcus elongatus sp. PCC 7942 or E.coli. Crude cell extract or a mix of co-purified polar compounds were offered as putative binding partners.
Project description:E.coli CutA was flow injected against cell extracts (cell pellets - of exponential growing cultures- have been extracted with 20% or 80% MeOH) of E.coli BW24113 and Synechococcus elongatus sp. PCC 7942 WT or deltacutA
Project description:Pulldown experiment with E.coli CutA. The protein was incubated for 2h with cell extracts of S. elongatus delta cutA or E. coli WT. Afterwards, the protein was precipitated with ethyl acetate. The organic phase was dried and resuspended in 50% MeOH before measurement.
Project description:Transcriptional profiling of E.coli SE15 comparing wild type E.coli SE15 with Autoindecur 2 synthesis gene LuxS mutnat E.coli SE15. E.coli SE15 is isolated from indwelling catheter of urinary tract infected patient. Examine change of quorum sensing related gene by deleting autoinducer 2 synthesis gene LuxS in E.coli