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The development of a high-throughput kinase activity platform using nanoLC-MS/MS with DIA approach for studying the anticancer mechanism of Taxol in ovarian cancer

ABSTRACT: Protein phosphorylation, a process mediated by protein kinases, plays a pivotal role in increasing protein diversity, thereby influencing various cellular functions. Peptide substrates have been employed for in vitro phosphorylation experiments using purified kinases or cell lysates. In this study, we have developed a quantitative, high-throughput platform for assessing multikinase activity, based on nanoLC-MS/MS with data-independent acquisition (DIA) approach. This platform was evaluated by studying the kinase activity of Taxol- treated SKOV3 cells. A library containing 38 peptide substrates was designed and analyzed to indicate the activity of major kinases in cancer development.

INSTRUMENT(S): maXis II

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Chao-Jung Chen

PROVIDER: MSV000093899 | MassIVE | Sun Jan 21 23:57:00 GMT 2024

REPOSITORIES: MassIVE

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