Proteomics

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Acetamiprid effect on queens and emerging bees


ABSTRACT: We continuously exposed honey bee colonies to sublethal acetamiprid doses (20 ug/L) under isolated conditions. After one month of exposure, the emerging bees and queens were collected and analyzed via high-throughput proteomics. The following treatment groups were established: i) a control without acetamiprid and ii) an acetamiprid-exposed group, in which the sugar solution was supplemented with acetamiprid to a final concentration of 20 ug/L. Six emerging bees were analyzed per colony. Each queen homogenate was processed three times for proteomic analysis. The data were acquired using the Data Independent Acquisition (DIA) method with an MS1 scan covering the range 390-1000 m/z at 120 K resolution (at 200 m/z), and the AGC target was set to 250%. and maximum injection time in Auto mode. DIA windows covered the range 390-1000 m/z in a total of 19 scans with 1 Da overlap and variable window width , Orbitrap resolution set to 30000, AGC target set to 1000% and maximum injection time in auto mode. Fragmentation was performed by HCD with a normalized collision energy of 30.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Apis Mellifera (ncbitaxon:7460)

SUBMITTER: Tomas Erban  

PROVIDER: MSV000093928 | MassIVE | Fri Jan 26 04:29:00 GMT 2024

REPOSITORIES: MassIVE

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