Ubiquitin remnant motif profiling (Ubiquitomics) in VACV infected HeLa cells

ABSTRACT: HeLa cells were pretreated with MG132, inoculated with VACV-Cop at an MOI of 3 for 1 h, and posttreated with MG132 again. Cells were lysed and extracted proteins were digested to peptides using trypsin, and desalted peptides were subjected to automated diGly enrichment using the PTMScan ubiquitin remnant motif kit (Cell signaling) according to manufactuer's instructions.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Vaccinia Virus Copenhagen (ncbitaxon:10249) Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Olivier Julien   Robert Ingham  

PROVIDER: MSV000094020 | MassIVE | Mon Feb 05 17:31:00 GMT 2024

REPOSITORIES: MassIVE