Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Host lipids regulate multicellular behavior of a predator of a human pathogen

ABSTRACT: Raw proteomics and lipidomics mass spectrometry data from Biomphalaria glabrata hemolymph samples. Hemolymph was collected by the foot-retraction method from: naive NMRI strains, naive M-line strains, or M-line strains infected and shedding PR1 Schistosoma mansoni. Untargeted proteomics analysis completed by the Laboratory for Biological Mass Spectrometry at Indiana University. Lipidomics analysis completed by the Beth Israel Deaconess Medical Center (BIDMC) Metabolomics Core in Boston, MA.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Schistosoma Mansoni (ncbitaxon:6183) Biomphalaria Glabrata (ncbitaxon:6526)

SUBMITTER: J.P. Gerdt

PROVIDER: MSV000094051 | MassIVE | Fri Feb 09 08:40:00 GMT 2024

SECONDARY ACCESSION(S): PXD049305

REPOSITORIES: MassIVE

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Biomphalaria Snail Hemolymph Lipidomics Upon Exposure to Schistosomes

Project description:Raw lipidomics mass spectrometry data from Biomphalaria glabrata hemolymph samples. Hemolymph was collected by the foot-retraction method from: naive NMRI strains, naive M-line strains, or M-line strains infected and shedding PR1 Schistosoma mansoni. Lipidomics analysis completed by the Beth Israel Deaconess Medical Center (BIDMC) Metabolomics Core in Boston, MA.

2024-02-12 | MSV000094067 | MassIVE

Project description:Biomphalaria glabrata hemolymph microbial sequences

| PRJNA1308600 | ENA

RNA-Seq analysis of Candida glabrata wild-type and Cgsnf2Δ strains in RPMI-grown or macrophage-internalized conditions at 2 h and 10 h post-infection.

Project description:The goal of the current study was to identify differentially-expressed genes upon CgSNF2 deletion, as well as, upon macrophage internalization in C. glabrata wild-type (wt) and Cgsnf2Δ strains. For this study, RNA samples were collected from RPMI-grown and macrophage-internalized cells of C. glabrata wild-type and Cgsnf2Δ strains at 2 h and 10 h post-infection. Comparative transcriptome analysis shows the differential regulation of 1419 genes in C. glabrata wild-type cells upon macrophage internalization, whereas the CgSNF2 deletion leads to altered regulation of 935 genes in C. glabrata wild-type cells.

2024-04-16 | GSE234670 | GEO

Limosilactobacillus fermentum Limits Candida glabrata Growth by Ergosterol Depletion

Project description:Candida glabrata is a human-associated opportunistic fungal pathogen. It shares its niche with Lactobacillus spp. in the gastrointestinal and vaginal tract. In fact, Lactobacillus species are thought to competitively prevent Candida overgrowth. We investigated the molecular aspects of this antifungal effect by analyzing the interaction of C. glabrata strains with Limosilactobacillus fermentum. From a collection of clinical C. glabrata isolates, we identified strains with different sensitivities to L. fermentum in coculture. We analyzed the variation of their expression pattern to isolate the specific response to L. fermentum. C. glabrata-L. fermentum coculture induced genes associated with ergosterol biosynthesis, weak acid stress, and drug/chemical stress. L. fermentum coculture depleted C. glabrata ergosterol. The reduction of ergosterol was dependent on the Lactobacillus species, even in coculture with different Candida species. We found a similar ergosterol-depleting effect with other lactobacillus strains (Lactobacillus crispatus and Lactobacillus rhamosus) on Candida albicans, Candida tropicalis, and Candida krusei. The addition of ergosterol improved C. glabrata growth in the coculture. Blocking ergosterol synthesis with fluconazole increased the susceptibility against L. fermentum, which was again mitigated by the addition of ergosterol. In accordance, a C. glabrata Derg11 mutant, defective in ergosterol biosynthesis, was highly sensitive to L. fermentum. In conclusion, our analysis indicates an unexpected direct function of ergosterol for C. glabrata proliferation in coculture with L. fermentum.

2023-02-08 | GSE202656 | GEO

Biofilm matrix regulation by Candida glabrata Zap1 under acidic conditions: transcriptomic and proteomic analyses

Project description:Transcriptional profiling of Candida glabrata zap1Δ and zap1Δ::ZAP1 strains, comparing cells grown under planktonic and biofilm-inducing conditions. Goal was to analyse the effect of ZAP1 deletion in the transcriptomic profile of C. glabrata biofilm cells (in comparision to planktonic cells), under acidic conditions, in order to study the function of the Zap1 transcription factor in C. glabrata biofilm matrix production.

2024-08-08 | GSE226915 | GEO

Differential transcriptomic responses of Biomphalaria glabrata to bacteria and metazoan parasites

Project description:Differential transcriptomic responses of Biomphalaria glabrata (Gastropoda, Mollusca) to bacteria and metazoan parasites Schistosoma mansoni and Echinostoma paraensei (Digenea, Platyhelminthes). Transcriptional profiling of Biomphalaria glabrata comparing control uninfected M-line B. glabrata with five experimental groups. The experimental groups are: wounded but not infected M-line, Escherichia coli infected, Micrococcus luteus infected, Echinostoma paraensei infected and Schistosoma mansoni infected at 12 hours time point post infection.

2009-09-01 | GSE16596 | GEO

Antifungal drug responses in two Candida glabrata strains

Project description:Transcriptomic responses of Candida glabrata strains, BG2 and CBS138, to two azole antifungal drugs, Fluconazole and Voriconazole.

2025-07-29 | GSE273379 | GEO

A comparative gene expression data set for functional Sfp1 knockdown mutants and wild type strains for five yeast Species in the LOG growth phase

Project description:This data set contains microarray data for wild type and Sfp1 mutant strains in each of several yeast species in the LOG growth phase. This was done at several time points (mainly the T=30, 60 or 90 min post glucose repletion) for S. cerevisiae, C. glabrata, N. castellii, K. lactis and S. pombe. For S. cerevisiae samples were taken for both WT and Sfp1 mutant conditions for both the BY and RM strains of this species. In S. cerevisiae, K. lactis and S. pombe there is one Sfp1 paralog and data were taken for the WT versions of these species and for a mutant in which Sfp1 was not functioning. This allows a comparison of the WT and mutant state in each species over several time points in the LOG growth phase. For S. cerevisiae both WT and Sfp1 mutant conditions were studied for both the BY and RM strains, as noted in the description of the samples and in the columns labels of the accompanying data matrix. For C. glabrata and N. Castellii there are two Sfp1 paralog genes, and data was taken for the individual mutant condition of each Sfp1 paralog in each species. These mutant data are noted with _9955 and _682 for the C. glabrata samples, and _719 and _511for the N. castellii samples. These designations indicate mutant conditions for either the CAGL0M09955g or CAGL0D00682g Sfp1 paralogs in C. glabrata, or likewise Scas719.24 or Scas551.1 in N. castellii. In the paper text N. castellii p719, C. glabrata p9995 are refered to as SFP1 and N. castellii p551 and C. glabrata paralog p0682 as SFP1PL.

2020-08-26 | GSE119645 | GEO

Project description:Hemolymph and hepatopancreas microbiomes of Biomphalaria glabrata snails during schistosome infection

| PRJNA1171869 | ENA

Differential transcriptomic responses of Biomphalaria glabrata to bacteria and metazoan parasites

2010-05-06 | E-GEOD-16596 | biostudies-arrayexpress