Proteomics

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Lacritin N-104 affinity purification of P. aeruginosa surface proteins


ABSTRACT: The precleared whole cell P. aeruginosa PA14 lysates underwent an overnight incubation with bead-immobilized N-104 peptide (or C-95 as a negative control peptide) under physiological conditions. Subsequent steps included washes and a gradual step-gradient elution ranging from 50 to 500 mM KCl. The eluate obtained at 500 mM KCl was then analyzed using tandem mass spectrometry to identify PA14 proteins that bind to N-104.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Pseudomonas Aeruginosa Pa14 (ncbitaxon:652611)

SUBMITTER: Gordon W. Laurie  

PROVIDER: MSV000094086 | MassIVE | Wed Feb 14 10:57:00 GMT 2024

REPOSITORIES: MassIVE

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