Mannose-6-phosphate isomerase functional status shapes a rearrangement in the proteome and degradome of mannose-treated melanoma cells

ABSTRACT: Metabolic reprogramming is a ubiquitous feature of transformed cells, comprising one of the hallmarks of cancer and enabling neoplastic cells to adapt to new environments. Accumulated evidence reports on the failure of some neoplastic cells to convert mannose-6-phosphate into fructose-6-phosphate, thereby impairing tumor growth in cells displaying low levels of the enzyme mannose-6-phosphate isomerase (MPI). Thus, we performed functional analyses and profiled the proteome landscape and the repertoire of substrates of proteases (degradome) of melanoma cell lines with distinct mutational backgrounds submitted to the treatment with mannose. Our results suggest a significant rearrangement in the proteome and degradome of melanoma cells lines upon mannose treatment, including the activation of catabolic pathways (such as protein turnover) and differences in protein N-terminal acetylation. Such biological implications might reflect the metabolic rewiring caused by the functional status of MPI enzyme and may help target specific molecular players from affected signaling circuits in melanoma.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Andre Zelanis  

PROVIDER: MSV000094098 | MassIVE | Fri Feb 16 06:24:00 GMT 2024

REPOSITORIES: MassIVE