Experimental viral infections in honey bee queens in the field
Ontology highlight
ABSTRACT:
INSTRUMENT(S): timsTOF Pro
ORGANISM(S): Apis Mellifera (ncbitaxon:7460)
SUBMITTER: 
Leonard Foster
PROVIDER: MSV000094113 | MassIVE | Sun Feb 18 17:52:00 GMT 2024
REPOSITORIES: MassIVE
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Project description:We injected honey bee queens with a combination of BQCV&DWV-B, the same combination of virus that was inactivated with UV, or saline and monitored them in queen monitoring cages for 7 days before sacrificing them and performing proteomics on the hemolymph, ovaries, and eggs that were collected from the cages.
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Project description:Honey bee queens undergo dramatic behavioral (e.g., reduced sexual receptivity), physiological (e.g., ovary activation, ovulation, and modulation of pheromone production) and molecular changes after they complete mating. To elucidate how queen post-mating changes are influenced by seminal fluid, a non-spermatozoa-containing component of semen, we injected queens with semen or seminal fluid alone. We assessed queen sexual receptivity, ovary development, worker retinue response (which is influenced by queen pheromone production), and transcriptional changes in queen abdominal fat body and brain tissues. Injection with either seminal fluid or semen resulted in decreased sexual receptivity, increased attractiveness of queens to workers, and altered expression of several genes that are also regulated in naturally mated queens. The post-mating and transcriptional changes of queens receiving seminal fluid were not significantly different from queens treated with seminal fluid, suggesting that components in seminal fluid, such as seminal fluid proteins, are largely responsible for stimulating post-mating changes in queens.
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