Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Experimental viral infections in honey bee queens in queen monitoring cages

ABSTRACT: We injected honey bee queens with a combination of BQCV&DWV-B, the same combination of virus that was inactivated with UV, or saline and monitored them in queen monitoring cages for 7 days before sacrificing them and performing proteomics on the hemolymph, ovaries, and eggs that were collected from the cages.

INSTRUMENT(S): timsTOF Pro

ORGANISM(S): Apis Mellifera (ncbitaxon:7460)

SUBMITTER: Leonard Foster

PROVIDER: MSV000094114 | MassIVE | Sun Feb 18 17:56:00 GMT 2024

REPOSITORIES: MassIVE

ACCESS DATA

Json Xml

Similar Datasets

Experimental viral infections in honey bee queens in the field

Project description:We injected honey bee queens with a either a combination of BQCV & DWV-B or saline and assessed them weekly in colonies for 7 weeks before sacrificing them and performing proteomics on the hemolymph and ovaries.

2024-02-18 | MSV000094113 | MassIVE

Indirect queen exposure to insect growth regulator pesticides

Project description:We exposed honey bee queens to insect growth regulator pesticides (IGRs) by feeding workers contaminated food. Queens and workers were housed in queen monitoring cages developed by Fine et al. and queen quality metrics were measured over the course of two weeks. At the end of this period, the queens were euthanized and their ovaries analyzed by shot-gun proteomics.

2022-04-01 | MSV000089189 | MassIVE

Phenotypic plasticity shapes genome architecture in the honeybee Apis mellifera (ChIP-seq)

Project description:Female honeybees are specified as workers or queens based on diet during early development. Workers are essentially sterile with a reduced number of ovarioles and no spermatheca. In the presence of the queen (queen mandibular pheromone) and her brood, worker ovaries are kept in an inactive quiescent state. If the queen is removed, or lost, worker bees are able to sense this change in their environment and their ovaries undergo complete remodelling producing unfertilised haploid eggs that will produce male (drone bees). In this study we analyse gene expression in queen, worker, and laying worker ovaries using RNA-seq and explore differences in the chromatin landscape (focussing on H3K27me3).

2019-09-17 | GSE120560 | GEO

Phenotypic plasticity shapes genome architecture in the honeybee Apis mellifera (RNA-seq)

Project description:Female honeybees are specified as workers or queens based on diet during early development. Workers are essentially sterile with a reduced number of ovarioles and no spermatheca. In the presence of the queen (queen mandibular pheromone) and her brood, worker ovaries are kept in an inactive quiescent state. If the queen is removed, or lost, worker bees are able to sense this change in their environment and their ovaries undergo complete remodeling producing unfertilized haploid eggs that will produce male (drone bees). In this study we analyze gene expression in queen, worker, and laying worker ovaries using RNA-seq and explore differences in the chromatin landscape (focusing on H3K27me3).

2019-09-17 | GSE120561 | GEO

Gut microbiota-driven regulation of queen bee ovarian metabolism

Project description:With the global prevalence of Varroa mites, more and more beekeepers resort to confining the queen bee in a queen cage to control mite infestation or to breed superior and robust queen bees. However, the impact of such practices on the queen bee remains largely unknown. Therefore, we subjected the queen bees to a 21-d egg-laying restriction treatment (from the egg stage to the emergence of adult worker bees) and analyzed the queen bees' ovarian metabolites and gut microbiota after 21 d, aiming to assess the queen bee's quality and assist beekeepers in better hive management. Our findings revealed a significant reduction in the relative expression levels of Vg and Hex110 genes in the ovaries of egg-laying-restricted queen bees compared to unrestricted egg-laying queens. The diversity of gut microbiota in the queen bee exhibited a notable decrease, accompanied by corresponding changes in the core bacterial species of the microbial community. Following egg-laying restriction, the activity of the queen bee's ovaries decreased, while the metabolism of glycerophospholipids remained or stored more lipid molecules, awaiting environmental changes for the queen bee to resume egg-laying promptly. Furthermore, we observed that Bombella in the queen bee's gut may regulate the queen's ovarian metabolism through tryptophan metabolism. These findings provide novel insights into the interplay among queen egg-laying, gut microbiota, and ovarian metabolism.

2023-09-11 | MTBLS7576 | MetaboLights

Regulation of Brain Gene Expression in Honey Bees by Queen Mandibular Pheromone

Project description:Expression profiling of honey bee brains exposed to queen mandibular pheromone. Exposure was performed in cages for 3 and 4 days; 3 and 4 days-old bees were analyzed

2011-09-01 | E-TABM-1102 | biostudies-arrayexpress

Comparison of Apis mellifera workers and queens brain development at fourth instar larvae

Project description:In Apis mellifera, the female eggs can develop into workers or queen depending on the diet offered during early development. The outputs of the developed honeybee females are two morphs with particular morphological traits and related physiology. The differential feeding regime experienced by the queen and the worker larvae of the honeybee Apis mellifera shapes a complex endocrine response cascade that ultimately sets up differences in brain morphologies. Herein we report on aspects of brain morphogenesis during larval development and the brain gene expression signature of fourth instar larvae (L4) of both castes, a developmental stage characterized by the greatest differences in juvenile hormone (JH) titers between castes Using results from the hybridization of whole genome-based oligonucleotide arrays with RNA samples from brain of fourth instar larvae honeybees of both castes we present a list of differentially expressed genes. Analysis used one dye-swap combination to compare workers and queens brain development at fourth instar larvae when juvenile hormone titers is higher in queens.

2012-12-31 | E-GEOD-39239 | biostudies-arrayexpress

Transcriptome sequencing of queen and virgin queen of two ants: Camponotus floridanus and Harpegnathos saltator

Project description:Deep sequencing of mRNA from queen and virgin queen of two Ants: Camponotus floridanus and Harpegnathos saltator

2012-08-09 | GSE31346 | GEO

smRNA sequencing of queen and virgin queen of two ants: Camponotus floridanus and Harpegnathos saltator

Project description:Deep sequencing of smRNA from queen and virgin queen of two Ants: Camponotus floridanus and Harpegnathos saltator

2012-08-09 | GSE31344 | GEO

Brain transcriptome of honeybees (Apis mellifera) infected by Nosema ceranae and/or Black Queen Cell Virus

Project description:Experimental infection of (2 days old) adult honey bee workers (30 bees per replicates, 3 replicates per treatments, from 3 different colonies (one colony per cage for each treatment)) with 10^9 genome equivalent of Black Queen Cell Virus (BQCV) in 10µl of sugar solution and/or 10^5 fresh Nosema ceranae spores (control bees were given a similar bee extract in PBS, without pathogen). Bees were kept in cages of 30 bees in incubator (30°C/50%RH). At day 13 p.i., bees were flash frozen, and stored at -80°C.

2016-09-06 | GSE81664 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data