Dataset Information

Astrocyte and neuron cell surface proteomes

ABSTRACT: We developed, tested and validated a method to determine the cell surface proteomes (CSPs) of striatal astrocytes and neurons. We used this method to study the CSPs and how they change in mouse models of disorders that affect the striatum. We compared our data to postmortem human data to identify disease-relevant mechanisms.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Baljit Khakh

PROVIDER: MSV000094132 | MassIVE | Tue Feb 20 11:09:00 GMT 2024

REPOSITORIES: MassIVE

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Publications

The cell-surface shared proteome of astrocytes and neurons and the molecular foundations of their multicellular interactions.

Wu Ling L Pandey Vijaya V Casha Vanessa H VH Qu Zhe Z Jami-Alahmadi Yasaman Y Gradinaru Viviana V Wohlschlegel James A JA Khakh Baljit S BS

Neuron 20250610 16

Neurons and astrocytes are predominant brain cells that extensively interact, but the molecular basis of their interactions remains largely unexplored. We identified and mapped striatal astrocytic and neuronal cell-surface proteins (CSPs) and found that many were shared, representing the cell-surface shared proteome of astrocytes and neurons (CS SPAN) bridging striatal astrocyte-neuron interaction sites. CS SPAN was replete with extracellular matrix proteins, cell adhesion molecules, transporter ...[more]

PMID: 40499536

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