Proteomics

Dataset Information

0

The NAE1-mediated Neddylation operates as an essential post-translational modification checkpoint for effector CD8+ T cells

ABSTRACT: Cells were lysed with TFA, and precipitated with cold acetone. The precipitates were transferred to E3filters and digestion was performed overnight with trypsin. The peptides were desalted with StageTips, and were analyzed on Eclipse Orbitrap with DIA mode. For MS1, the resolution was set to 120,000. Mass range was 380-980 m/z. AGC target was standard. Max injection time was auto. For MS/MS, the isolation window was 8 m/z. Activation type was HCD with 30% NCE. The scan range was 145-1450 m/z. The normalized AGC target was 800%. Max injection time was auto. For FAIMS, three CVs were used, -40v, -55v, and -75v.

INSTRUMENT(S): Orbitrap Eclipse

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Gang Xin  

PROVIDER: MSV000094179 | MassIVE | Tue Feb 27 08:22:00 GMT 2024

REPOSITORIES: MassIVE

Json Xml

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1
altmetric image

Publications

The NAE1-mediated neddylation operates as an essential post-translational modification checkpoint for effector CD8<sup>+</sup> T cells.

Jin Jiacheng J   Zhang Ruohan R   Li Jianying J   Gao Fengxia F   Liao Zhiwei Z   Yu Yanbao Y   Wang Yi Y   Bucci Donna D   Xiao Min M   Ma Ruilin R   Ma Qin Q   Gao Shuaixin S   Lio Jerry J   Novais Fernanda F   Huang Stanley Ching-Cheng SC   Zhu Jiangjiang J   Ghoneim Hazem H   Wen Haitao H   Li Zihai Z   Sun Nuo N   Xin Gang G  

Proceedings of the National Academy of Sciences of the United States of America 20250303 10

Optimal activation of CD8+ T cells is crucial for immunity-mediated destruction of cancer, requiring a substantial amount of proteins involved in metabolism, proliferation, and effector function. Despite extensive studies emphasizing the role of transcriptional regulation in this process, paired transcriptomic and proteomic analyses reveal that the RNA profile is poorly correlated with protein levels. This discrepancy underscores the importance of post-translational modifications (PTMs) in contr  ...[more]

Similar Datasets

Proteomics Acetamiprid effect on queens and emerging bees
2024-01-26 | MSV000093928 | MassIVE
Genomics Exosomal miRNA profile of BNCT treated glioblastoma cells
2024-04-16 | GSE243221 | GEO
Proteomics Presynaptic Rac1 Interactome in vivo BioID Soderling and Kim
2024-06-21 | MSV000095110 | MassIVE
Proteomics Proteogenomic analysis of hiPSC cultures from a healthy donor
2024-07-03 | MSV000095226 | MassIVE
Proteomics mHtt tobacco roots proteome, QEHFX
2019-11-07 | MSV000084557 | MassIVE
Genomics
| PRJNA464174 | ENA
Proteomics VCP enhances autophagy-related osteosarcoma progression by recruiting USP2 to inhibit ubiquitination and degradation of FASN
2024-09-10 | PXD055763 |
Transcriptomics Chronic stress targets mitochondrial respiratory efficiency in the skeletal muscle of C57BL/6 mice [Transcriptome Dataset]
2023-04-03 | GSE210365 | GEO
Transcriptomics Early and advanced gastric cancer genomes
2014-06-30 | GSE57176 | GEO
Transcriptomics Liposome transfection of miR-145 induces immune response via RIG-I in mesenchymal stem cells
2012-10-01 | E-GEOD-40387 | biostudies-arrayexpress