Methylobacterium sp. Leaf119 LC-MS Data
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Xevo G2-S QTof
ORGANISM(S): Methylobacterium Sp. Leaf119 (ncbitaxon:1736261)
SUBMITTER: 
Aaron Puri
PROVIDER: MSV000094181 | MassIVE | Tue Feb 27 09:58:00 GMT 2024
REPOSITORIES: MassIVE
Similar Datasets
Project description:Raw LC-MS/MS data of crude extract of Methylobacterium sp. Leaf119. Included are .mzml and .raw files for a 12C-methanol blank, 13C-methanol blank, 13C-methanol plus 12C-PABA, 13C-methanol plus methionine, 13C-methanol plus both PABA and methionine, and 12C-methanol plus deuterated methionine (methyl-D3).
2024-04-30 | MSV000094644 | MassIVE
Project description:Raw LC-MS/MS data of crude extract of Methylobacterium sp. Leaf119. Included are .mzml and .raw files for a 12C-methanol blank, 13C-methanol blank, 13C-methanol plus 12C-PABA, 13C-methanol plus methionine, 13C-methanol plus both PABA and methionine, and 12C-methanol plus deuterated methionine (methyl-D3).
2024-04-30 | MSV000094644 | GNPS
Project description:Raw LC-MS/MS data of crude extract of Methylobacterium sp. Leaf119. Included are .mzml and .raw files for a 12C-methanol blank, 13C-methanol blank, 13C-methanol plus 12C-PABA, 13C-methanol plus methionine, and 13C-methanol plus both PABA and methionine.
2024-02-27 | MSV000094181 | GNPS
Project description:Engineered Methylobacterium methanol batch exponential
2014-12-22 | GSE34662 | GEO
Project description:Engineered Methylobacterium methanol batch exponential strain CM702 vs. strain CM502
2014-12-22 | E-GEOD-34662 | biostudies-arrayexpress
Project description:Methylobacterium extorquens AM1 on methanol
2012-02-28 | PRD000446 | Pride
Project description:Differential analysis of Methylobacterium extorquens DM4 in methanol versus dichloromethane condition using shotgun label free MS1 quantification approach
2018-04-25 | PXD006838 | Pride
Project description:In order to provide information about the gene expression response that occurs when cells experience a change in carbon source, succinate limited chemostat cultures of Methylobacterium extorquens AM1 were grown to and maintained at an OD of ~0.63, transferred to flasks and methanol was added. Cells were harvested for RNA extraction at time: 0 min, 10 min, 30 min, 1 hr, 2 hr, 4 hr and 6 hr post transition. At 30 min, a no methanol addition sample was extracted as a carbon starvation control. These data were used in conjunction with flux, enzymatic and metabolite measurements to assess the changes in central metabolism during this transition. Abstract from manuscript: When organisms experience environmental change, how does their metabolic network reset and adapt to the new condition? This study focused on the mechanisms of metabolic adaptation occurring during the transition from succinate to methanol growth by the methylotrophic bacterium Methylobacterium extorquens, analyzing changes in carbon flux, gene expression, metabolites and enzymatic activities over time. Initially, cells experienced metabolic imbalance with excretion of metabolites, changes in nucleotide levels and cessation of cell growth. Though assimilatory pathways were induced rapidly, a transient block in carbon flow to biomass synthesis occurred, and enzymatic assays suggested methylenetetrahydrofolate dehydrogenase as one control point. This “downstream priming” mechanism ensures that significant carbon flux through these pathways does not occur until they are fully induced, precluding the buildup of toxic intermediates. Most metabolites that are required for growth on both carbon sources did not change significantly, even though transcripts and enzymatic activities required for their production changed radically, underscoring the concept of metabolic setpoints.
2011-08-01 | GSE22031 | GEO
Project description:This dataset has been used to establish GroEL-SIP, coupling a targeted proteotyping approach for assessing bacterial community compositions using the taxonomic marker protein GroEL with stable isotope probing to link the identified taxa to substrate assimilation. This dataset contains raw data of four experiments: 1.) Pure cultures of T. aromatica cultivated with 13C or 12C benzoate mixed in defined ratios. 2.) Pure cultures of P. putida cultivated with 13C or 12C benzoate mixed in a 1:1 ratio. 3.) Pure cultures of E. coli cultivated with 12C acetate and 13C or 12C benzoate mixed in a 1:1 ratio. 4.) Co-cultivated biculture of T. aromatica and P. putida cultivated with 13C or 12C benzoate and mixed in a 1:1 ratio. 5.) Co-cultivated biculture of T. aromatica and E. coli cultivated with 12c acetate and 13C or 12C benzoate and mixed in a 1:1 ratio. 1)-3) were analyzed after in-solution digestion 4)-5) were analyzed after in-gel digestion of the 60 kDa band
2024-10-17 | PXD048255 | Pride
Project description:In order to provide information about the gene expression response that occurs when cells experience a change in carbon source, succinate limited chemostat cultures of Methylobacterium extorquens AM1 were grown to and maintained at an OD of ~0.63, transferred to flasks and methanol was added. Cells were harvested for RNA extraction at time: 0 min, 10 min, 30 min, 1 hr, 2 hr, 4 hr and 6 hr post transition. At 30 min, a no methanol addition sample was extracted as a carbon starvation control. These data were used in conjunction with flux, enzymatic and metabolite measurements to assess the changes in central metabolism during this transition. Abstract from manuscript: When organisms experience environmental change, how does their metabolic network reset and adapt to the new condition? This study focused on the mechanisms of metabolic adaptation occurring during the transition from succinate to methanol growth by the methylotrophic bacterium Methylobacterium extorquens, analyzing changes in carbon flux, gene expression, metabolites and enzymatic activities over time. Initially, cells experienced metabolic imbalance with excretion of metabolites, changes in nucleotide levels and cessation of cell growth. Though assimilatory pathways were induced rapidly, a transient block in carbon flow to biomass synthesis occurred, and enzymatic assays suggested methylenetetrahydrofolate dehydrogenase as one control point. This “downstream priming” mechanism ensures that significant carbon flux through these pathways does not occur until they are fully induced, precluding the buildup of toxic intermediates. Most metabolites that are required for growth on both carbon sources did not change significantly, even though transcripts and enzymatic activities required for their production changed radically, underscoring the concept of metabolic setpoints. Gene expression in succinate limited chemostat cultures was compared to gene expression in cells transferred to flasks before and after methanol addition. As a control, a time = 0 sample (RNA prepared from cells harvested directly from the chemostat) was compared to a time = 0 sample immediately obtained after the cells were transferred to flasks, before methanol was added in order to identify changes due to flask transfer. A carbon starvation control was also done comparing expression from time = 0 (chemostat cells) to cells transferred to flasks for 30 min with no carbon source added. Two biological replicates each with two techinal replicates (dye swap) were analyzed for time = 0 (chemostat) vs 10 min, 30 min, 1 hr and 2 hr after methanol addition. One biological replicate with two technical replicates (dye swap) were analyzed for time = 0 (chemostat) vs time = 0 (flask transfer), and time = 0 (chemostat) vs time = 4 hr, 6 hr and 30 min no methanol addition.
2011-07-31 | E-GEOD-22031 | biostudies-arrayexpress