Multivalent Coiled-Coil Interactions as a Conserved Design Principle for Centrosome Assembly

ABSTRACT: To enrich for monomeric SPD-5 (in Storage Buffer; 25 mM HEPES, pH 7.4, 500 mM KCl, 0.1% CHAPS, 1% glycerol) was first centrifuged at 80,000 rpm for 10 min at 4 C to remove possible aggregates, then diluted in Assembly Buffer (25 mM HEPES, pH 7.4, 150 mM KCl) + 8mM DMTMM for 15 min. Reactions were quenched with 50 mM ammonium bicarbonate and incubated at 23 C for 15 min.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Caenorhabditis Elegans (ncbitaxon:6239)

SUBMITTER: Jeffrey Woodruff  

PROVIDER: MSV000094193 | MassIVE | Wed Feb 28 11:30:00 GMT 2024

REPOSITORIES: MassIVE