Proteomics

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Multivalent Coiled-Coil Interactions as a Conserved Design Principle for Centrosome Assembly


ABSTRACT: Purified GFP-CDK5RAP2 (in Storage Buffer; 25 mM HEPES, pH 7.4, 500 mM KCl, 0.1% CHAPS, 1% glycerol) was diluted in Assembly Buffer (25 mM HEPES, pH 7.4, 150 mM KCl) to achieve a final protein concentration of 1 mM protein and final KCl concentration of 250mM. Samples were incubated at room temperature for 2 hr to allow formation of oligomeric assemblies. Final concentrations of 8 mM DMTMM (Sigma-Aldrich) were added to the protein samples and incubated at 23 C with shaking for 45 min. Reactions were quenched with 50 mM ammonium bicarbonate and incubated with shaking at room temperature for 15 min.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Caenorhabditis Elegans (ncbitaxon:6239)

SUBMITTER: Jeffrey Woodruff  

PROVIDER: MSV000094195 | MassIVE | Wed Feb 28 13:45:00 GMT 2024

REPOSITORIES: MassIVE

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