DATASET - Bottom-Up - Snake venom proteomics of seven taxa of the genera Vipera, Montivipera, Macrovipera and Daboia across Turkiye/Turkey

ABSTRACT: This DATASET collection includes the mass spectrometry files for proteomics venom investigation of seven taxa of the genera Vipera, Montivipera, Macrovipera and Daboia across Turkiye/Turkey. Species list: Vipera berus barani Vipera darevskii Montivipera bulgardaghica bulgardaghica Montivipera bulgardaghica albizona Montivipera xanthina Macrovipera lebetinus obtusa Daboia palaestinae Folders 01-07 - BOTTOM-UP PROTEOMICS: The venom pools were investigated by the bottom-up "snake venomics" (labled as SVX) approach and in short: separated by RP-HPLC, followed by SDS-PAGE separation and the single bands were in-gel processed by DTT, IAC and finally o/n tryptic digested. Samples submitted to HPLC-MS/MS. Early peptidic fractions of the first HPLC run were directly submitted to HPLC-MS/MS analytic w/o further gel procession. Folders 01 to 07 include the MS and MS/MS spectra of the snake species 1-7, respectively. Files are included as RAW and MZML format. Used instrument: LTQ Orbitrap XL mass spectrometer (Thermo, Bremen, Germany) with an Agilent 1260 HPLC system (Agilent Technologies, Waldbronn, Germany) using a reversed-phase Grace Vydac 218MS C18 (2.1 x 150 mm; 5 um particle size) column. Modifications: UNIMOD:4 - \"Iodoacetamide derivative.\"

INSTRUMENT(S): LTQ Orbitrap XL

ORGANISM(S): Montivipera Xanthina (ncbitaxon:110207) Vipera Barani (ncbitaxon:247077) Daboia Palaestinae (ncbitaxon:1170828) Macrovipera Lebetina (ncbitaxon:8709) Vipera Berus Barani Montivipera Albizona (ncbitaxon:110210) Macrovipera Lebetinus Vipera Darevskii (ncbitaxon:2493147) Montivipera Bulgardaghica (ncbitaxon:1850566) Macrovipera Lebetina Obtusa (ncbitaxon:209528) Montivipera Bulgardaghica Albizona Vipera Berus (ncbitaxon:31155) Montivipera Bulgardaghica Bulgardaghica

SUBMITTER: Maik Damm  

PROVIDER: MSV000094228 | MassIVE | Mon Mar 04 00:14:00 GMT 2024

REPOSITORIES: MassIVE