Minimal Change Disease: a proteomics approach

ABSTRACT: This study aimed to shed light on the potential pathophysiology of MCD by using glomerular proteomic analysis. Shotgun proteomics using label-free quantitative mass spectrometry was performed on formalin-fixed, paraffin-embedded (FFPE) renal biopsies from two groups of samples: control (CTR) and MCD. Glomeruli were excised from FFPE renal biopsies using laser capture microdissection (LCM), and a single-pot solid-phase-enhanced sample preparation (SP3) digest method was used to improve yield and protein identifications.

INSTRUMENT(S): SYNAPT G2-Si

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Iuga Cristina-Adela  

PROVIDER: MSV000094293 | MassIVE | Tue Mar 12 01:54:00 GMT 2024

REPOSITORIES: MassIVE