Affinity Purification Mass Spectrometry of protein protein interactors with alternatively-spliced Uromodulin

ABSTRACT: We identified an alternatively spliced UMOD which is uniquely localized intracellularly. This intracellular isoform enhanced mitochondrial function. To understand its molecular mechanism, we performed affinity-purification coupled to mass spectrometry using MDCK cell lysate expressing UMOD variant. We prepared MDCK cells which express mock (negative control), wild-type human UMOD or alternatively spliced human UMOD. UMOD was isolated by Immunoprecipitation and interacting proteins were identified by mass spectrometry proteomics analysis.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Canis Lupus Familiaris (ncbitaxon:9615) Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Tarek M. El-Achkar   Amber L. Mosley  

PROVIDER: MSV000094329 | MassIVE | Fri Mar 15 12:52:00 GMT 2024

SECONDARY ACCESSION(S): PXD050667

REPOSITORIES: MassIVE