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Identification and characterization of novel pathogen antigens and epitopes and their impact on self MHC class I immunopeptidomes by applying PEPSeek software

ABSTRACT: Analysis of the pathogen-derived peptides presented via MHC class I pathway of infected cells is a cutting-edge strategy to identify epitope candidates suitable for vaccine development. This dataset contains raw data from mass spectrometry measurements of the immunopeptidomes of HeLa cells infected with Chlamydia trachomatis and mouse macrophage cells infected with Listeria monocytogenes along with cognate control files, search results from standard search engine approaches, and PEPSeek outputs with pathogen epitopes identified. We further provide search engine and PEPSeek outputs for reanalysis of data from immunopeptidomes of multiple human cell lines infected SARS-COV2 and Listeria monocytogenes. Finally, we include raw files and analysis of files for synthetic peptide measurements of a sample of pathogen epitopes identified which provided validation of the identified peptides through spectral comparison.

INSTRUMENT(S): Orbitrap Fusion Lumos, Orbitrap Fusion

ORGANISM(S): Chlamydia Trachomatis (ncbitaxon:813) Severe Acute Respiratory Syndrome Coronavirus 2 (ncbitaxon:2697049) Listeria Monocytogenes (ncbitaxon:1639) Homo Sapiens (ncbitaxon:9606) Mus Musculus (ncbitaxon:10090)

SUBMITTER: Michele Mishto Dr. Juliane Liepe

PROVIDER: MSV000094354 | MassIVE | Tue Mar 19 02:02:00 GMT 2024

REPOSITORIES: MassIVE

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