Project description:Investigation of whole genome expression pattern of 60 and 72 hours post fertilization Danio Rerio embryos exposed to TMT and vehicle control Embryos were exposed to 10uM TMT or control from 48hpf to 60 or 72 hpf. Three replicates were collected for each time point. 40 embryos were pooled to comprise a replicate.
Project description:All samples were gathered from mouse RAW 264.7 cells (macrophages). Control total RNA was extracted from untreated RAW 264.7 cells cultured for either 1, 2, 4, 8, 16 or 48 hours. Test total RNA was extracted from lipopolysaccharide (100ng/ml) and lipopolysaccharide-binding protein (100pM) treated RAW 264.4 cells cultured for either 1, 2, 4, 8, 16 or 48 hours. This SuperSeries is composed of the following subset Series: GSE1099: Effect of LPS and LPS-binding protein treatment for 1 hour on RAW 264.4 cells GSE1100: Effect of LPS and LPS-binding protein treatment for 2 hours on RAW 264.4 cells GSE1101: Effect of LPS and LPS-binding protein treatment for 4 hours on RAW 264.4 cells GSE1102: Effect of LPS and LPS-binding protein treatment for 8 hours on RAW 264.4 cells GSE1103: Effect of LPS and LPS-binding protein treatment for 16 hours on RAW 264.4 cells GSE1104: Effect of LPS and LPS-binding protein treatment for 48 hours on RAW 264.4 cells Refer to individual Series
Project description:Investigation of whole genome expression pattern of 60 and 72 hours post fertilization Danio Rerio embryos exposed to TMT and vehicle control
Project description:Gram-negative bacterial infections can cause varying degrees of liver injury in chickens. Although andrographolide has been shown to have a protective effect on the liver, its underlying mechanism of action and effects on liver proteins are not known. The study objectives were to analyze the actions of andrographolide on lipopolysaccharide (LPS)-induced chicken leghorn male hepatoma (LMH) cell injury, identify the different proteins in different groups using TMT proteomics, and explore the pharmacological effects and potential targets of andrographolide in LPS-induced liver injury. It was found that andrographolide reduced alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels in the cell supernatant and alleviated LPS-induced injury in LMH cells. Proteomic analysis identified 50 and 166 differentially expressed proteins in the LPS vs NC group and LPS-Andro vs LPS group, respectively. Andrographolide may be involved in steroid metabolic processes, negative regulation of MAPK cascade, oxidative stress, and other processes to protect against LPS-induced liver injury. HMGCS1, HMGCR, FDPS, PBK, CAV1, PRDX1, PRDX4, and PRDX6, which were identified by differential proteomics, may be the targets of andrographolide. Our study may provide new theoretical support for andrographolide protection against liver injury.
Project description:A. simplex s.s. (parasitic nematode) under the in vitro treatment with different concentrations of lipopolysaccharide (LPS) of Escherichia coli. Complete TMT10plex labeling. LC-MS/MS analysis.
Project description:To investigate the effect of maternal inflammation during pregnancy on placenta, we injected LPS to pregnant dams at embryonic day 14 and analyzed gene expression.
Project description:To investigate the possible impact of MOK in microglial immune responses, we tested the effect of LPS treatment in MOK-KO (H14) vs. WT SIM-A9 cells.