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NCI-H226 TEAD4 FLAG AP-MS profiling

ABSTRACT: The goal of this experiment is to identify changes in TEAD4 protein interactions upon treatment with TEAD small molecule inhibitors. NCI-H226 cells were treated for 24h with DMSO vehicle control or a TEAD inhibitor, GNE-7883 or Compound 2. Immunoprecipitation of TEAD4 was performed and the resulting protein samples were processed for tandem mass tag (TMT) labeling and quantitative proteomic analysis. Pooled TMT-labeled samples were fractionated into six fractions and the experiment was performed in triplicate. For further experimental details, see the Materials and Methods section in the manuscript.

INSTRUMENT(S): Orbitrap Eclipse

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Jennie Lill

PROVIDER: MSV000094638 | MassIVE | Mon Apr 29 17:07:00 BST 2024

REPOSITORIES: MassIVE

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