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Label-free quantification of proteomic changes during C2C12 myoblast differentiation

ABSTRACT: Global proteome comparison between five cell populations isolated during a 4-day differentiation process in C2C12 myoblasts. A total of 5 replicates were in-column digested and data was collected in data independent acquisition mode on a Lumos Tribrid instrument for label free-quantification of protein abundances. Day0 population= Myoblasts, Day1 populations= live cells and dead cells, Day4 populations= Myotubes and Reserve cells.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Olivier Julien

PROVIDER: MSV000094656 | MassIVE | Wed May 01 16:57:00 BST 2024

REPOSITORIES: MassIVE

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An atlas of caspase cleavage events in differentiating muscle cells.

Gomez-Cardona Erik E Dehkordi Mahshid H MH Van Baar Kolden K Vitkauskaite Aiste A Julien Olivier O Fearnhead Howard O HO

Protein science : a publication of the Protein Society 20240901 9

Executioner caspases, such as caspase-3, are known to induce apoptosis, but in other contexts, they can control very different fates, including cell differentiation and neuronal plasticity. While hundreds of caspase substrates are known to be specifically targeted during cell death, we know very little about how caspase activity brings about non-apoptotic fates. Here, we report the first proteome identification of cleavage events in C2C12 cells undergoing myogenic differentiation and its compari ...[more]

PMID: 39180494

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