Proteomics

Dataset Information

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MI-AEG-TKK-YL-JSB_15LFQ_ARL5A_ARL5B


ABSTRACT: Label free mass spectrometry analysis to identify potential effectors of the ARL5A and ARL5B GTPases. During the MitoID proximity biotinylation experiment, bait constructs were made by fusing sequences encoding a mitochondrial-targeting sequence (MTS) from the outer mitochondrial membrane protein TOM20, along with the biotin ligase BioID2, to the N-terminus of constitutively active (Q70L) or inactive (T30N) forms of human ARL5A or ARL5B lacking the N-terminal amphipathic helix. MTS-BioID2 was used as a negative control.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Juan Bonifacino  

PROVIDER: MSV000094675 | MassIVE | Sat May 04 20:02:00 BST 2024

SECONDARY ACCESSION(S): PXD051988

REPOSITORIES: MassIVE

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