Single cell proteomics of an HDAC inhibitor by label free proteomics
Ontology highlight
ABSTRACT: NCI-H-358 cancer cells were treated with the HDAC inhibitor mocetinostat for 24 hours. Single cells were isolated by FACs and prepared in a single well prior to diaPASEF analysis on a TIMSTOF SCP. A 40cm column was used for all analysis using a 200nL/min active gradient of 180 minutes. Library runs were created by pooling 16 single control and treated cells and running on the same gradient (separately) and processed with SpectroNaut 18.
INSTRUMENT(S): timsTOF SCP
ORGANISM(S): Homo Sapiens (ncbitaxon:9606)
SUBMITTER:
Benjamin C Orsburn
PROVIDER: MSV000094678 | MassIVE | Sun May 05 14:51:00 BST 2024
SECONDARY ACCESSION(S): PXD051994
REPOSITORIES: MassIVE
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