Proteomics

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Single cell proteomics of an HDAC inhibitor by label free proteomics


ABSTRACT: NCI-H-358 cancer cells were treated with the HDAC inhibitor mocetinostat for 24 hours. Single cells were isolated by FACs and prepared in a single well prior to diaPASEF analysis on a TIMSTOF SCP. A 40cm column was used for all analysis using a 200nL/min active gradient of 180 minutes. Library runs were created by pooling 16 single control and treated cells and running on the same gradient (separately) and processed with SpectroNaut 18.

INSTRUMENT(S): timsTOF SCP

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Benjamin C Orsburn  

PROVIDER: MSV000094678 | MassIVE | Sun May 05 14:51:00 BST 2024

SECONDARY ACCESSION(S): PXD051994

REPOSITORIES: MassIVE

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