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An algicidal bacterium shapes the microbiome during outdoor diatom cultivation collapse

ABSTRACT: Proteomic measurements for samples from 5 outdoor algal ponds over a time series; ponds were sampled during the fall of 2015. Samples were digested with trypsin, then analyzed by LC-MS/MS. Data was searched with MS-GF+ using PNNL's DMS processing pipeline against FASTA files compiled from ponds sequenced metagenomes and proteomes of dominating algae Nannochloropsis salina and Phaeodactylum tricornutum.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Phaeodactylum Tricornutum (ncbitaxon:2850) Nannochloropsis Salina (ncbitaxon:5749)

SUBMITTER: Ty Samo

PROVIDER: MSV000094933 | MassIVE | Tue Jun 04 17:53:00 BST 2024

REPOSITORIES: MassIVE

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A bloom of a single bacterium shapes the microbiome during outdoor diatom cultivation collapse.

Gilbert Naomi E NE Kimbrel Jeffrey A JA Samo Ty J TJ Siccardi Anthony J AJ Stuart Rhona K RK Mayali Xavier X

mSystems 20250514 6

Algae-dominated ecosystems are fundamentally influenced by their microbiome. We lack information on the identity and function of bacteria that specialize in consuming algal-derived dissolved organic matter in high algal density ecosystems such as outdoor algal ponds used for biofuel production. Here, we describe the metagenomic and metaproteomic signatures of a single bacterial strain that bloomed during a population-wide crash of the diatom, <i>Phaeodactylum tricornutum,</i> grown in outdoor po ...[more]

PMID: 40366134

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