Dataset Information

Proteomic investigation of seed development under high nitrate supply in plants lacking Pyrroline-5-carboxylate dehydrogenase

ABSTRACT: Free proline is an amino acid known to have many functions in plants, particularly in stress adaptation and development. Its metabolism is well known, but its exact role in plant physiology has remained elusive. Proline catabolism is ensured by the sequential action of the enzymes Proline dehydrogenase (ProDH) and Pyrroline-5-carboxylate dehydrogenase (P5CDH). In this study, seed development in the p5cdh mutant was investigated under low and high nitrate supply. It was found that, in p5cdh mutants, embryo development was blocked at the transition between embryogenesis and the maturation phase. As a result, the whole maturation phase was disturbed, starting with a shorter photosynthetic phase, followed by a lower accumulation of storage compounds but a higher accumulation of proline when compared to wild-type embryos. Finally, at the end of seed development, mutant embryos appeared to be unable to tolerate desiccation and died during this final stage of development. This phenotype was strongly enhanced under high nitrate. Together with multiomics analysis, these data show that proline catabolism is important for redox homeostasis and energy supply during seed maturation. This dataset contains the respective proteomic data of the project.

INSTRUMENT(S): timsTOF Pro

ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702)

SUBMITTER: Hans-Peter Braun Nils Rugen

PROVIDER: MSV000094979 | MassIVE | Mon Jun 10 23:48:00 BST 2024

SECONDARY ACCESSION(S): PXD052996

REPOSITORIES: MassIVE

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Publications

Pyrroline-5-carboxylate dehydrogenase is a key actor of nitrogen metabolism in maturing seeds of Arabidopsis thaliana.

Dourmap Corentin C Baud Sébastien S Eubel Holger H Rugen Nils N Crilat Émilie É Beradocco Solenne S Marnet Nathalie N Clément Gilles G Tcherkez Guillaume G Planchais Séverine S Cabassa Cécile C Lebreton Sandrine S Bordenave-Jacquemin Marianne M Maldiney Régis R Carol Pierre P Braun Hans-Peter HP Masclaux-Daubresse Céline C Bouchereau Alain A Savouré Arnould A Guivarc'h Anne A

Journal of experimental botany 20260112

Proline is a multifaceted amino acid in plants involved in both stress responses and development. Recent studies have shown that knock-out mutants lacking Pyrroline-5-carboxylate dehydrogenase (P5CDH), the enzyme responsible for the second step of proline catabolism, impaired nitrogen remobilisation and carbon allocation to seeds. Here, we demonstrate that seed development is also significantly impaired in p5cdh mutants, particularly from the transition between embryogenesis and maturation. Spec ...[more]

PMID: 41521765

Similar Datasets

Project description:In comparison with provision of either ammonium or nitrate alone, simultaneously supplying both forms of N results in superior growth and yield for the majority of plants including rice. Using a rice 22K oligo-array, we performed transcriptome analysis to identify genes of rice (Oryza sativa L. ssp. japonica) responsive to change of N-supply forms and N-starvation. Using the supply of ammonium nitrate (one to one molar ratio) as control, the total number of root genes that were equal or more than two fold up- or down- regulated was 445, 324, and 781 by upon supply of either ammonium or nitrate or continuous N starvation, respectively for 96 h. In the shoot the equivalent numbers were much smaller only 32, 58, and 165, respectively. Clustering of the rice genes associated with different environmental stresses revealed substantial organ specificity of the root and shoot to N starvation, and also to the N supply form. Genes encoding transporters for ammonium and nitrate, nitrate reductase, glutamate dehydrogenase, and aspartate amino transferase, showed great response to change of the N supply form, especially to N starvation. Some of the genes involved in chlorophyll metabolism, carbon fixation and assimilation, were enhanced by ammonium supply only, but significantly suppressed by N-starvation. In the shoot there was increased expression of more general stress genes under nitrate when compared to ammonium nutrition. In the root the reverse situation was true with more apparent stress under ammonium nutrition. The microarray approach has revealed new levels of complexity in the response of rice to the form of N supply. Experiment Overall Design: Rice seedlings were grown in a hydroponic system in a growth chamber with control of both temperature and light. After normal growth for two weeks and for one week with completely avoiding any N source, the plants at four and a half leaf stage were re-supplied with ammonium nitrate (equal amount of either form of N), or only ammonium (ammonium sulphate and ammonium chloride), or only nitrate (calcium nitrate and magnesium nitrate), and continuing at zero N (N starvation). All the other essential nutrients were the same for all four treatments, except for sulfate and chloride which ranged between 1.1 â 2.6 mM and 0.5 â 2.5 mM, respectively. In Arabidopsis, addition of 3 mM Cl to âcontrolsâ did not produce any changes in the expression of genes in an experiment investigating the re-supply of 3 mM nitrate (Scheible et al., 2004). In other microarray experiments, extra addition of 5 mM Cl for Arabidopsis (Wang et al., 2000; Wang et al., 2004) and 1.4 mM sulphate for tomato (Wang et al., 2001) were used to replace nitrate for identifying the N deprivation responsive genes. Therefore, we assume that the relative smaller difference in sulfate and chloride concentrations among the four treatments in the normal supply range for plants would not significantly affect the expression profile of genes responsible for changes in N supply form and starvation in our experiments. Experiment Overall Design: For identifying the genes responding to the various N conditions by micro-array analysis, we extracted total RNA respectively from the roots and shoots at the 96 h after initiation of the four respective treatments. We used amplified and labeled cRNA from ammonium nitrate treatment as control, we performed array-hybridization for the cRNA from the treatment of single ammonium form, single nitrate form, or continuous N depletion, respectively. Agilent 60-mer oligonucleotide arrays containing 21,938 unique transcription units (Agilent Technologies, Tokyo, Japan) were used. Two biological replicates of each treatment were performed for microarray analyses.