Project description:This experiment uses denaturing crosslinking IP of Muskelin in wildtype Drosophila embryos to enrich for close-proximity interactions.
Project description:Chromatin immunopurifications from heat shocked drosophila embryos with anti-Heat shock factor sera compared with immunopurification with pre-immune sera. Hybridisations to cDNA arrays. Keywords: repeat sample
Project description:In order to analyze the global changes in gene expression resulting from loss of Fra signaling, we performed a microarray experiment comparing Drosophila embryos containing a loss of function fra[3] mutation to age matched wildtype embryos. RNA extracted from fra[3] mutant vs. wildtype embryos were hybridized to the Affymetrix GeneChip Drosophila Genome 2.0 .
Project description:This study uses individual-nucleotide resolution UV crosslinking and immunoprecipitation (iCLIP) to map the direct RNA-binding sites of the PETISCO complex in Caenorhabditis elegans embryos. When bound to TOST-1, PETISCO stabilizes replication-dependent histone mRNAs during oogenesis to ensure adequate maternal histone supply for early embryogenesis. To identify the direct RNA targets of PETISCO, we performed iCLIP using anti-TOFU-6 antibodies in embryos. This approach revealed highly specific enrichment of replication-dependent histone transcripts, with crosslinking sites concentrated in the 3' regions of these mRNAs, particularly 12 nucleotides upstream of the conserved stem-loop structure.
Project description:Expression, crosslinking and immunoprecipitation of tagged HNRPA2B1 (variant A2) to identify bound RNA sequences RNA molecules that are immunoprecipitated are sequenced followed purification under denaturing conditions
Project description:Proteins co-purifying with recombinantly purified and N-terminally GFP-3C-tagged CTCF N-terminus (amino acids 1-293) produced in bacteria were identified by incubating the tagged bait with soluble nuclear protein extracts from 0-12h hour-old wildtype (OregonR strain) Drosophila melanogaster embryos.
Project description:Chromatin immunopurifications from heat shocked drosophila embryos with anti-Heat shock factor sera compared with immunopurification with pre-immune sera. Hybridisations to cDNA arrays.