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Flap endonuclease 1 repairs DNA-protein crosslinks via ADP-ribosylation

ABSTRACT: The protein composition of DNA-protein crosslinks (DPCs) induced by formaldehyde treatment of human cell lines was analyzed by mass spectrometry.

INSTRUMENT(S): Orbitrap Fusion Lumos, Orbitrap Fusion, Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Lisa Jenkins

PROVIDER: MSV000095039 | MassIVE | Fri Jun 14 09:00:00 BST 2024

SECONDARY ACCESSION(S): PXD053127

REPOSITORIES: MassIVE

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Flap endonuclease 1 repairs DNA-protein cross-links via ADP-ribosylation-dependent mechanisms.

Sun Yilun Y Jenkins Lisa M LM El Touny Lara H LH Zhu Linying L Yang Xi X Jo Ukhyun U Escobedo Lauren L Maity Tapan K TK Saha Liton Kumar LK Uribe Isabel I Saha Sourav S Takeda Shunichi S Leung Anthony K L AKL Cheng Ken K Pommier Yves Y

Science advances 20250110 2

DNA-protein cross-links (DPCs) are among the most detrimental genomic lesions. They are ubiquitously produced by formaldehyde (FA), and failure to repair FA-induced DPCs blocks chromatin-based processes, leading to neurodegeneration and cancer. The type, structure, and repair of FA-induced DPCs remain largely unknown. Here, we profiled the proteome of FA-induced DPCs and found that flap endonuclease 1 (FEN1) resolves FA-induced DPCs. We revealed that FA also damages DNA bases adjoining the DPCs, ...[more]

PMID: 39792662

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CSB promotes transcription-coupled repair of DNA-protein crosslinks

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2024-02-15 | PXD047668 | Pride

Interactome analysis of elongating RNAPII after induction of DNA damage by IP-MS/MS

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Atypical K6-linked ubiquitylation marks toxic RNA-protein crosslinks induced by formaldehyde

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Atypical K6-linked ubiquitylation marks toxic RNA-protein crosslinks induced by formaldehyde

Project description:Atypical K6-linked ubiquitylation marks toxic RNA-protein crosslinks induced by formaldehyde

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DNA sequence at CpG islands directly influences chromatin structure via the CpG binding protein Cfp1

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