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Raw LFQ-MS data, profiling of hFWE4-positive vesicles and hFWE4-mediated alterations to cell surface proteome in epidermal keratinocytes

ABSTRACT: LFQ-MS profiling of hFWE4-positive vesicles and hFWE4-mediated alterations to cell surface proteome in epidermal keratinocytes

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Laura A. Hansen

PROVIDER: MSV000095056 | MassIVE | Mon Jun 17 11:53:00 BST 2024

REPOSITORIES: MassIVE

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Publications

Flower dependent trafficking of lamellar bodies facilitates maturation of the epidermal barrier.

Rudd Justin C JC Smits Jos P H JPH Kuwong Patrick T PT Johnson Rachel E RE Monga Louise M N LMN van Vlijmen-Willems Ivonne M J J IMJJ Porter Greer L GL Halloran Peter O PO Sharma Kanika K Schmidt Karina N KN Kumar Vikas V Madson Justin G JG Sarkar Mrinal K MK van den Bogaard Ellen H EH Grunkemeyer James A JA Gudjonsson Johann E JE Wong Sunny Y SY Simpson Cory L CL Hansen Laura A LA

Nature communications 20250726 1

Specialized secretory cells, including keratinocytes in the last viable layers of mammalian epidermis, utilize lysosome-related organelles (LROs) to exocytose distinct cargoes vital for tissue function. Here, we demonstrate that the Flower isoform, hFWE4, a putative Ca<sup>2+</sup> channel that permits endocytic retrieval of presynaptic vesicles and lytic granules, also resides on epidermal lamellar bodies (LBs), an LRO that extrudes a proteinaceous lipid-rich matrix to finalize the epidermal ba ...[more]

PMID: 40715079

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