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Broadening the horizon of MS optimization for enzymatic digestions in proteomics

ABSTRACT: Collision energy dependent measurements on human protein exctract and human blood plasma samples digested with various enzymes (trypsin, Arg-C, Glu-C, chymotrypsin, Asp-N) and performance assessment at optimized energies on Bruker QTof instruments

INSTRUMENT(S): maXis

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Agnes Revesz

PROVIDER: MSV000095066 | MassIVE | Tue Jun 18 08:51:00 BST 2024

REPOSITORIES: MassIVE

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Publications

The Enzyme Effect: Broadening the Horizon of MS Optimization to Nontryptic Digestion in Proteomics.

Nagy Kinga K Sándor Péter P Vékey Károly K Drahos László L Révész Ágnes Á

Journal of the American Society for Mass Spectrometry 20250113 2

In recent years, alternative enzymes with varied specificities have gained importance in MS-based bottom-up proteomics, offering orthogonal information about biological samples and advantages in certain applications. However, most mass spectrometric workflows are optimized for tryptic digests. This raises the questions of whether enzyme specificity impacts mass spectrometry and if current methods for nontryptic digests are suboptimal. The success of peptide and protein identifications relies on ...[more]

PMID: 39803703

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Files for: Improved Protein Inference from Multiple Protease Bottom-Up Mass Spectrometry Data

Project description:LC-MS/MS raw files for Jurkat cell lysate aliquots digested with either Arg-C, Asp-N, Chymotrypsin, Glu-C, Lys-C or Trypsin and fractionated offline to produce 11 fractions (Trypsin only has 10 fractions).

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The impact II, a very high resolution quadrupole time-of-flight instrument for deep shotgun proteomics

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Chlorpyrifos oxon crosslinking of amyloid beta 42 peptides is a new route for generation of self-aggregating amyloidogenic oligomers that promote Alzheimer’s disease

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Effect of trmA on tRNA expression in Pseudomonas aeruginosa

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2023-01-17 | PXD037491 | Pride

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