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Deep Profiling of Plasma Proteoforms with Engineered Nanoparticles for Top-down Proteomics

ABSTRACT: Raw data for the manuscript entitled "Deep Profiling of Plasma Proteoforms with Engineered Nanoparticles for Top-down Proteomics"

INSTRUMENT(S): Orbitrap Eclipse

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Neil L Kelleher

PROVIDER: MSV000095086 | MassIVE | Wed Jun 19 12:44:00 BST 2024

REPOSITORIES: MassIVE

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Publications

Deep Profiling of Plasma Proteoforms with Engineered Nanoparticles for Top-Down Proteomics.

Huang Che-Fan CF Hollas Michael A MA Sanchez Aniel A Bhattacharya Mrittika M Ho Giang G Sundaresan Ambika A Caldwell Michael A MA Zhao Xiaoyan X Benz Ryan R Siddiqui Asim A Kelleher Neil L NL

Journal of proteome research 20240923 10

The dynamic range challenge for the detection of proteins and their proteoforms in human plasma has been well documented. Here, we use the nanoparticle protein corona approach to enrich low-abundance proteins selectively and reproducibly from human plasma and use top-down proteomics to quantify differential enrichment for the 2841 detected proteoforms from 114 proteins. Furthermore, nanoparticle enrichment allowed top-down detection of proteoforms between ∼1 μg/mL and ∼10 pg/mL in absolute abund ...[more]

PMID: 39312774

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