Enhanced payload localization in antibody-drug conjugates using a middle-down mass spectrometry approach with proton transfer charge reduction
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ABSTRACT: Here, we present an MD MS strategy relying on the use of PTCR to investigate an ADC with variable drug-to-antibody ratio, targeting the unambiguous localization of payload conjugation sites. Unlike traditional tandem MS experiments (MS2), which were largely ineffective, a single PTCR-based experiment (MS3) proved to be sufficient to achieve this goal across all modified ADC subunits.
INSTRUMENT(S): Orbitrap Ascend
ORGANISM(S): Antibody Drug Conjugate
SUBMITTER:
Luca Fornelli
PROVIDER: MSV000095128 | MassIVE | Mon Jun 24 09:26:00 BST 2024
REPOSITORIES: MassIVE
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