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Metallosphaera sedula bifurcates into two sizes when it is cultured mixotrophically on soluble iron

ABSTRACT: In situ kinetic measurements were conducted on Metallosphaera sedula as it respired aerobically on extracellular soluble iron. Cells cultured on the same organic nutrients in the absence or the presence of soluble iron exhibited the same level of respiratory activity, suggesting that the responsible proteins were expressed constitutively. The unexpected and novel observation was that cells cultured in the presence of iron comprised equal numbers of two types of cells, one larger and one smaller than the relatively uniform cells that were cultured in the absence of iron. Proteomic studies were conducted on cell-free extracts of cells that were cultured in the absence or the presence of soluble iron. The only relevant proteomic observation was that the enzymes of the TCA cycle were expressed in higher numbers in the cells that were cultured in the absence of soluble iron.

INSTRUMENT(S): LTQ Orbitrap Velos, Q Exactive Plus

ORGANISM(S): Metallosphaera Sedula (ncbitaxon:43687)

SUBMITTER: Robert C. Blake II

PROVIDER: MSV000095146 | MassIVE | Tue Jun 25 14:40:00 BST 2024

REPOSITORIES: MassIVE

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