Understanding the genetic and structural bases of hornworts' carbon-concentrating mechanism

ABSTRACT: Proteomic measurements to identify putative Rubisco interacting proteins in hornworts compared to the green alga Chlamydomonas, the traditional model for studying pyrenoid-based CO2-concentrating mechanisms (pCCM). Using antibodies and immunoprecipitation against hornwort Rubisco large subunits to pull down Rubisco and any proteins that bind to it. Samples were digested with trypsin, then analyzed by LC-MS/MS. Data was searched with MS-GF+ using PNNL's DMS processing pipeline against Anthoceros agrestis proteome.

INSTRUMENT(S): Orbitrap Eclipse

ORGANISM(S): Anthoceros Agrestis (ncbitaxon:41834)

SUBMITTER: Fay-Wei Li  

PROVIDER: MSV000095322 | MassIVE | Fri Jul 12 15:57:00 BST 2024

SECONDARY ACCESSION(S): PXD053926

REPOSITORIES: MassIVE