Alkylation reageant comparaison

ABSTRACT: Comparison of off target alkylation using chloroacetamide (CAA) and iodoacetamide (IAA). The peptide preparation was performed side by side, following identical methods except the alkylation treatment with either IAA or CAA. None of the comparison of off-targets on between IAA and CAA showed a significant difference. The overall off-target level for both reagents was relatively low (< 0.1%) and the off-target rate on lysine residues was even lower (0.045% for IAA and 0.051% for CAA).

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702)

SUBMITTER: Justin Walley  

PROVIDER: MSV000095495 | MassIVE | Wed Jul 31 08:58:00 BST 2024

SECONDARY ACCESSION(S): PXD054447

REPOSITORIES: MassIVE