Heart surfaceome profiling using biotin capture

ABSTRACT: Cell surface proteins (surfaceome) represent key signalling and interaction molecules for therapeutic targeting, biomarker profiling, and cellular phenotyping. Understanding the surfaceome of complex tissues including the heart is essential to investigate membrane composition and function in physiological and pathological states. Here, we present a protein-centric workflow that enables the labelling, capture and detection of the cardiac cell surfaceome using ultra-high-sensitivity mass spectrometry-based proteome analysis in individual murine hearts. By preserving cardiac cell architecture of the neo-native heart, this workflow combines coronary artery perfusion using cleavable biotin and unbiased capture of the surface-accessible heart proteome.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: David Greening  

PROVIDER: MSV000095912 | MassIVE |

REPOSITORIES: MassIVE