LFQ DIA to analyze the proteome of NPC mutant iNeurons with and without ferric ammonium citrate
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ABSTRACT: Label-free quantification (LFQ) using data-independent acquisition (DIA) on the Orbitrap Astral was performed to analyze the proteome of iNeurons with NPC1 and NPC2 knocked out. The experiment includes the following time points of development: day 0, 4, 8, 16, and 22. All samples were cultured in regular media, with or without ferric ammonium citrate (FAC), and processed in triplicates for each time point, genotype, and treatment.
INSTRUMENT(S): Orbitrap Astral
ORGANISM(S): Homo Sapiens (ncbitaxon:9606)
SUBMITTER:
J. Wade Harper
Joshua J. Coon
PROVIDER: MSV000095985 | MassIVE |
SECONDARY ACCESSION(S): PXD056356
REPOSITORIES: MassIVE
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