Dataset Information

Circadian clock control of ribosome composition promotes rhythmic translation and termination fidelity

ABSTRACT: We describe circadian clock-dependent changes in ribosome composition in Neurospora crassa depending on cell type, stress, or developmental state. Mass spectrometry of ribosomes isolated at different circadian times identified six ribosomal proteins and one ribosome-associated protein with clock-controlled abundance. We confirmed clock control of eL31-HA abundance in purified ribosomes and found that deletion of eL31 altered and inhibited translation rhythms. We examined ribosome protected footprint (RPF)-seq reads mapping past stop codons over circadian time to reveal clock-dependent and eL31-enhanced rhythms in translation termination fidelity. We discovered that eL31 promotes proper ion homeostasis and translation elongation fidelity, and demonstrated that the circadian clock governs daily changes in ribosome composition that control rhythms in translation and impact translation fidelity, likely through changes in intracellular Mg2+ levels.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Neurospora Crassa (ncbitaxon:5141)

SUBMITTER: Deborah Bell-Pedersen