De novo peptide sequencing tools benchmark

ABSTRACT: Our study is a large-scale evaluation of de novo peptide sequencing tools using both publicly and proprietary heterogeneous datasets spanning multiple species, mass spectrometers, post-translational modifications, and a wide range of use cases. This dataset contains a publicly available subset of this dataset. The dataset includes raw MS/MS spectra in their original vendor formats as well as in mzML and Mascot Generic Format (MGF). Additionally, peptide spectral hits (PSMs) identified through a database search and rescoring workflow, along with the dataset-specific protein databases used for annotation, are provided.

INSTRUMENT(S): LTQ Orbitrap, Orbitrap Fusion ETD, LTQ Orbitrap Velos, Q Exactive HF-X, TripleTOF 5600+, Orbitrap Astral, Orbitrap Fusion, Orbitrap Exploris 480, timsTOF Pro, Orbitrap Fusion Lumos, 6550 iFunnel Q-TOF LC/MS, Q Exactive HF, Q Exactive

ORGANISM(S): Bacillus Subtilis (ncbitaxon:1423) Candidatus Thiodiazotropha Endoloripes (ncbitaxon:1818881) Salmonella Enterica (ncbitaxon:28901) Escherichia Coli (ncbitaxon:562) Vigna Mungo (ncbitaxon:3915) Solanum Lycopersicum (ncbitaxon:4081) Homo Sapiens (ncbitaxon:9606) Saccharomyces Cerevisiae (ncbitaxon:4932) Mus Musculus (ncbitaxon:10090) Methanosarcina Mazei (ncbitaxon:2209) Apis Mellifera (ncbitaxon:7460)

SUBMITTER: Wout Bittremieux  

PROVIDER: MSV000096182 | MassIVE | Wed Oct 23 15:40:00 BST 2024

REPOSITORIES: MassIVE