Proteomics

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SysQuan: repurposing SILAC mice for affordable absolute quantitation of the human proteome


ABSTRACT: We have repurposed SILAC mouse tissues/biofluids as system-wide internal standards for matched human samples to enable absolute quantitation (AbsQuan) of theoretically >2/3 of the human proteome using 132,380 shared tryptic peptides. We demonstrate that SysQuan enables quantifying 70% and 31% of the largest liver and plasma reference proteomes, respectively. We exemplify for 14 metabolic proteins that abundant standard isotope labeled mouse tissues enable cost-effective reverse AbsQuan in - theoretically 1000s of - human samples. Moreover, 10,000s of light/heavy doublets in untargeted SysQuan datasets enable unique post-acquisition AbsQuan.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (ncbitaxon:9606) Mus Musculus (ncbitaxon:10090)

SUBMITTER: Rene Zahedi  

PROVIDER: MSV000096198 | MassIVE | Thu Oct 24 10:23:00 BST 2024

REPOSITORIES: MassIVE

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