Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Untargeted metabolomics in seeds of Arabidopsis wild-type, TT7 KO and other flavonoid mutants

ABSTRACT: Seeds of Arabidopsis thaliana wild type and knockout-mutants for flavonoid genes (tt7 [3 lines], fls1, tt3, tt4, tt7tt4 [2 lines] ) were subjected to untargeted metabolomic analyses (LC-MS/MS) in positive and negative ionisation mode.

INSTRUMENT(S): impact II

ORGANISM(S): Arabidopsis Thaliana (ncbitaxon:3702)

SUBMITTER: Massimiliano Corso Sophie Jasinski

PROVIDER: MSV000096214 | MassIVE | Fri Oct 25 05:50:00 BST 2024

REPOSITORIES: MassIVE

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

RNA-seq of ΔDS deletion vs +WT Complemented seeds

Project description:We identified a regulator of Arabidopsis thaliana seed germination: FLOE1 (AT4G28300). We used RNA-seq to uncover genes that are differentially regulated in dry seeds, imbibed seeds and seeds imbibed in 220mM NaCl in mutant lines complemented with a ΔDS deletion version of FLOE1 (ΔDS) compared to mutants complemented with a WT version of FLOE1 (+WT).

2021-06-28 | GSE167291 | GEO

Differentially expressed genes in developing seeds of Arabidopsis thaliana accessions Col-0 and C24

Project description:In order to identify differentially expressed genes in developing seeds of Arabidopsis thaliana three different stages of seed development were analysed (9-10, 10-11 and 12-13 days after flower opening) for two Arabidopsis thaliana accessions, Col-0 and C24. For each stage and accession three biological replicates were analysed.

2017-05-21 | GSE94762 | GEO

Transcriptional profiles underlying parent-of-origin effects in seeds of Arabidopsis thaliana

Project description:This SuperSeries is composed of the following subset Series: GSE20005: Parent-of-origin effects in seeds of Arabidopsis thaliana: Affymetrix GSE20006: Parent-of-origin effects in seeds of Arabidopsis thaliana: Agilent Refer to individual Series

2010-08-26 | E-GEOD-20007 | biostudies-arrayexpress

Dormancy heterogeneity among Arabidopsis thaliana seeds is linked to individual seed size

Project description:Production of morphologically and physiologically variable seeds is an important strategy that helps plants to survive in unpredictable natural conditions. However, the model plant Arabidopsis thaliana and most agronomically essential crops yield visually homogenous seeds. Using automated phenotype analysis, we observed that in Arabidopsis small seeds tend to have higher primary and secondary dormancy levels when compared to large ones. Transcriptomic analysis revealed distinct gene expression profiles between large and small seeds. Large seeds had higher expression of translation-related genes implicated in germination competence. In contrast, small seeds showed elevated expression of many positive regulators of dormancy, including a key regulator of this process – the DOG1 gene. Differences in DOG1 expression were associated with differential production of its alternative cleavage and polyadenylation isoforms where in small seeds proximal poly(A) site is selected resulting in a short mRNA isoform. Furthermore, single-seed RNA-seq analysis demonstrated that large seeds resemble DOG1 knockout mutant seeds. Finally, on the single seed level, the expression of genes affected by seed size was correlated with the expression of genes positioning seeds on the path towards germination. Our results demonstrate an unexpected link between seed size and dormancy phenotypes in a species producing highly homogenous seed pools, suggesting that the correlation between seed morphology and physiology is more widespread than initially assumed.

2023-10-16 | GSE203310 | GEO

RNA-seq of floe1-1 vs Col-0 seeds

Project description:We identified a regulator of Arabidopsis thaliana seed germination: FLOE1 (AT4G28300). We used RNA-seq to uncover genes that are diffenrentially regulated in dry seeds, imbibed seeds and seeds imbibed in 220mM NaCl.

2021-06-28 | GSE167280 | GEO

Re-establishment of desiccation tolerance by PEG in germinated Arabidopsis thaliana seeds

Project description:Mature seeds of Arabidopsis thaliana are desiccation tolerant, but they lose DT while progressing to germination. Yet, there is a small developmental window during which DT can be rescued by treatment with polyethylene glycol (PEG). We used microarrays to identify relevant genes in the re-establishment of desiccation tolerance by PEG.

2015-08-25 | GSE64227 | GEO

Transcriptional characterization of Arabidopsis thaliana seeds treated with a red- or far red-light pulse

Project description:The goal of this study was to compare the transcriptional profile (RNA-seq) of imbibed Arabidopsis thaliana Columbia-0 ecotype seeds that were treated with a 20 min red or far red pulse. The red-light pulse induces germination.

2019-07-10 | GSE134019 | GEO

transcriptome and translatome of arabidopsis wt seeds according to dormancy-transcriptomic and translatomic analysis of Arabidopsis seeds

Project description:au13-12_polysome - transcriptome and translatome of arabidopsis wt seeds according to dormancy - Identification of transcripts that are differentially abundant (transcriptome) and transcripts that are addressed to translation (translatome) in imbibed Arabidopsis seeds in relation with dormancy. During imbibition of seeds (16h and 24h in darkness at 25°C, dormant and non-dormant seeds), transcriptome analysis is done on total RNA and translatome analysis on polysomal RNA. - At harvest seeds are dormant. They stay dormant if they are stored at -20°C (D) and become non-dormant (ND) if they are stored 3 weeks at +20°C. Arabidopsis dormant seeds do not germinate at 25°C in darkness while non-dormant seeds do. Total RNA and polysomal RNA (polysomal fractions purified on sucrose gradients) were extracted from imbibed seeds for 16h or 24h at 25°C in darkness (3 biological replicates). Transcriptome and translatome are compared for Dormant vs Non-Dormant for 16h and 24 imbibition. In silico comparison will allow to compare transcriptome and translatome for each point and type of seeds and to compare the time points (16 vs 24h) for each type of sample.

2014-09-27 | GSE61809 | GEO

Proteomics Analysis of the UMK2 Mutation in Arabidopsis thaliana seeds

Project description:We compared the proteome of dry as well as germinating seeds of Arabidopsis thaliana wild type Col-0 with the respective proteomes from an umk2 (AT4G25280) frameshift mutant. Germinating seeds were incubated for 48 hours in 1/2-MS medium in Petri dishes at 4 degree Celsius followed by 24 hours of a phytochamber under long-day conditions. Protein extraction, digestion by SP3 workflow, and LC-IMS-MS/MS analysis as described in https://doi.org/10.1038/s41477-022-01308-6.

2023-07-03 | MSV000092331 | MassIVE