Small molecule-induced alterations of protein polyubiquitination revealed by mass-spectrometric ubiquitome analysis

ABSTRACT: Mammalian cells were treated with different small molecules which interfere with the ubiquitin system. After cell lysis, ubiquitinated proteins were enriched with biotin-tagged TUBE, digested with Trypsin/LysC and labelled with TMT. Peptides were fractionated at high pH before nano-LC-MS/MS analysis.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Petra Janning  

PROVIDER: MSV000096295 | MassIVE | Tue Nov 05 07:53:00 GMT 2024

SECONDARY ACCESSION(S): PXD057541

REPOSITORIES: MassIVE