Project description:An actinomycete, Streptomyces sp. PSRA5 was isolated from Panax sokpayensis, a medicinal plant of the Sikkim Himalayas. It was found to have antimicrobial activity against multi-drug resistant Staphylococcus aureus. The crude extract of the actinomycete was extracted and analysed.
Project description:Enzymatic Methyl-seq (EM-seq) is an enzyme-based method giving us reliable methylome data from small amounts of purified DNA. However, it is unclear whether EM-seq library can be constructed from crude cell lysate containing genomic DNA. We evaluated the quality of EM-seq libraries directly prepared from crude cell lysate.