Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Iron-starvation induces photosystem I antenna remodeling in green algae

ABSTRACT: Dunaliella salina and Dunaliella tertiolecta are extremophile, marine algae that can survive in very low Fe conditions. In this study, we used TMT-proteomics to compare the Fe starvation responses to the Fe replete responses. Samples were digested with trypsin, labeled with TMT 10-Plex, then analyzed by LC-MS/MS. Data was searched with MS-GF+ using PNNL's DMS Processing pipeline.

INSTRUMENT(S): Orbitrap Eclipse

ORGANISM(S): Dunaliella Tertiolecta (ncbitaxon:3047) Dunaliella Cf. Bardawil Chpd1 (ncbitaxon:1227098)

SUBMITTER: Sabeeha Merchant

PROVIDER: MSV000096479 | MassIVE | Wed Nov 20 19:32:00 GMT 2024

SECONDARY ACCESSION(S): PXD058109

REPOSITORIES: MassIVE

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Publications

Fe starvation induces a second LHCI tetramer to photosystem I in green algae.

Liu Helen W HW Khera Radhika R Grob Patricia P Gallaher Sean D SD Purvine Samuel O SO Nicora Carrie D CD Lipton Mary S MS Niyogi Krishna K KK Nogales Eva E Iwai Masakazu M Merchant Sabeeha S SS

bioRxiv : the preprint server for biology 20241212

Iron (Fe) availability limits photosynthesis at a global scale where Fe-rich photosystem (PS) I abundance is drastically reduced in Fe-poor environments. We used single-particle cryo-electron microscopy to reveal a unique Fe starvation-dependent arrangement of light-harvesting chlorophyll (LHC) proteins where Fe starvation-induced TIDI1 is found in an additional tetramer of LHC proteins associated with PSI in <i>Dunaliella tertiolecta</i> and <i>Dunaliella salina</i>. These cosmopolitan green al ...[more]

PMID: 39713434

Similar Datasets

The transcriptome of Tetraselmis suecica reveals new insights on green algae response to nitrogen starvation

Project description:Nitrogen starvation is an efficient environmental pressure used to increase lipid accumulation and oil droplet formation in microalgal cells. Various studies focused on metabolic changes occurring in microalgae in nitrogen starvation conditions, but the mechanisms at the basis of these changes are not completely understood. Between microalgae, green algae, with more than 7000 species growing in a variety of habitats, have been frequently studied for energy purposes, but also as source of bioactive extracts/compounds. In this study, de novo transcriptome of the green algae Tetraselmis suecica has been performed in order to (1) deeply study its response to nitrogen starvation, (2) to look for enzymes with antioxidant capacity and for polyketide synthases (PKSs), (3) if present, to evaluate if nutrient starvation can influence their expression levels.

2018-12-01 | GSE109461 | GEO

The human innate immune protein calprotectin elicits a multi-metal starvation response in Pseudomonas aeruginosa

Project description:To combat infections, the mammalian host limits availability of essential transition metals such as iron (Fe), zinc (Zn), and manganese (Mn) in a strategy termed “nutritional immunity”. The innate immune protein calprotectin (CP) contributes to nutritional immunity by sequestering these metals to exert antimicrobial activity against a broad range of microbial pathogens. One such pathogen is Pseudomonas aeruginosa, which causes opportunistic infections in vulnerable populations including individuals with cystic fibrosis. CP was previously shown to withhold Fe(II) and Zn(II) from P. aeruginosa and induce Fe- and Zn-starvation responses in this pathogen. In this work, we performed quantitative, label-free proteomics to further elucidate how CP impacts metal homeostasis pathways in P. aeruginosa. We report that CP induces an incomplete Fe-starvation response, as many Fe-containing proteins that are repressed by Fe limitation are not affected by CP treatment. The Zn-starvation response elicited by CP seems to be more complete than the Fe-starvation response and includes increases in Zn transporters and Zn-independent proteins. CP also induces the expression of membrane-modifying enzymes, and metal-depletion studies indicate this response results from the sequestration of multiple metals. Moreover, the increased expression of membrane-modifying enzymes upon CP treatment correlates with increased tolorance to polymyxin B. Thus, response of P. aeruginosa to CP treatment includes both single and multi-metal starvation responses and includes many factors related to virulence potential, broadening our understanding of this pathogen’s interaction with the host.

2021-07-29 | PXD027638 | Pride

Factors governing the transcriptome changes and chronological lifespan of fission yeast during phosphate starvation

Project description:This RNA-Seq analysis compares gene expression of the pho7∆ fission yeast (Schizosaccharomyces pombe) strain prior to phosphate starvation (0 HR), i.e. in phosphate replete conditions, and at various times after phosphate starvation (4, 8, 12, 24, 36, and 48 HR) contrasting to the WT fission yeast S. pombe cells grown in phosphate replete conditions (WT 0HR)

2024-01-27 | GSE247799 | GEO

Transcript level coordination of carbon pathways during silicon starvation induced lipid accumulation in the diatom Thalassiosira pseudonana

2015-12-31 | GSE75651 | GEO

Chlamydomonas reinhardtii responses to Fe-excess, Fe-deficiency, and Fe-limitation in either photoautotrophic or mixotrophic growth

Project description:A systems level analysis of Chlamydomonas reinhardtii grown photoautotrophically or mixotrophically with a reduced carbon source, acetate, under four different defined Fe stages of Fe-replete, Fe-deficient, Fe-limited, or Fe-excess. Samples were digested with trypsin, labeled with TMT 10-Plex, then analyzed by LC-MS/MS. Data was searched with MS-GF+ using PNNL's DMS Processing pipeline.

2024-11-20 | MSV000096475 | MassIVE

Synechocystis PCC6803 dose responses to iron starvation (96h).

Project description:The transcriptome approach was used to characterize the global change (2 Fe concentrations) in Synechocystis gene expression in response to continuous iron starvation, compared to standard medium (17 microM). For iron depletion, exponentially growing cells were washed in Fe-free medium, and grown for 48 hrs under standard conditions in liquid medium cotaining either 1 mM or 2 mM of ferric ammonium citrate. Then, cells were washed, and resuspended in Fe-free medium (Fe contaminations 0.5 microM) and grown for another 48 hrs period. For each concentration point, total RNA were isolated from stressed and unstressed cells, reverse-transcribed, differentially labelled (dye swapped), hybridized together (stressed versus unstressed samples) and analyzed with DNA glass microarrays (two slides per each concentration point) (Custom-commercial array : IntelliGeneTM CyanoCHIP version 2.0, TAKARA). To identify differentially expressed genes, the median of the normalized ratio of Cy5/Cy3 intensity was calculated for each spot of the replicated dye-swap. The results of the analysis were carefully examined to exclude the dye effect between the 2 Cy-swapped arrays. Keywords: stress responses

2005-12-07 | GSE3717 | GEO

Expression analysis of N. europaea (ATCC 19718) wildtype and pFur::Kan mutant grown in Fe-replete and Fe-limited media

Project description:Investigation of whole genome gene expression level changes in a Nitrosomonas europaea (ATCC 19718) wildtype and pFur::Kan mutant [kanamycin resistance cassette insertion in the promoter region of the fur gene (NE0616)] strains grown in Fe-replete and Fe-limited media. The Nitrosomonas europaea (ATCC 19718) wiltype cells grown in Fe-limited media were compared to cells grown in Fe-replete media to gain a better understanding of the metabolic changes occurring in response to iron stress. The Nitrosomonas europaea (ATCC 19718) pFur::Kan mutant strain grown in Fe-replete & Fe-limited media were compared to wildtype cells grown in Fe=replete & Fe-limited media to gain a better understanding of the role Fur (NE0616) plays in iron homeostasis control.

2009-11-10 | GSE18709 | GEO

Factors governing the transcriptome changes and chronological lifespan of fission yeast during phosphate starvation I

Project description:RNA-seq analysis compares gene expression of the fission yeast (Schizossacharomyces pombe) ecl123∆ mutant at various times after phosphate starvation (4, 8, 12, 24, 36 and 48h) to the ecl123∆ mutant fission yeast strain grown in phosphate replete conditions (0h, prior to phosphate starvation).

2024-01-27 | GSE247922 | GEO

Transcript level coordination of carbon pathways during silicon starvation induced lipid accumulation in the diatom Thalassiosira pseudonana

Project description:Nutrient-starvation induced lipid accumulation has been reported in diverse algae, including diatoms. Molecular mechanisms underlying lipid accumulation in nutrient-starved algae are of interest to inform genetic engineering strategies aimed at improving lipid productivity. Diatom cell walls are made of nanostructured silica which is a unique feature of the group and silicon deprivation induces both growth arrest and lipid accumulation. In this work, we report the whole cell transcript level response during silicon starvation induced lipid accumulation. Analyzed mRNA from cells after 0, 4, 8, 12, 18, and 24 hr of silicon starvation using the Affymetrix GeneChip whole genome tiling array. Initial analysis of gene level expression was performed using the Affymetrix Expression Console Software, version 1.1. No biological replicates were performed. 0 hr is used as a reference point.

2015-12-31 | E-GEOD-75651 | biostudies-arrayexpress

Candida glabrata WT and mutants under iron starvation

Project description:Transcriptional time series of Candida glabrata under iron starvation (SD medium without Fe). Wild type and deletion mutants of the iron-related transcription factors Aft1 and Sef1, as well as of the iron uptake transporter Ftr1 as a positive control.

2016-10-05 | GSE84816 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data