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The proteome landscape of extracellular vesicles from prostate cancer cell lines

ABSTRACT: Proteome of extracellular vesicles (EVs) collected from prostate cancer cell lines - LNCaP, 22RV1, LNCaP 95, C4-2, DU145, PC3, NCI-H660, LASCPC-01, EF1.

INSTRUMENT(S): Orbitrap Eclipse

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Justin M. Drake PhD

PROVIDER: MSV000096519 | MassIVE | Mon Nov 25 13:46:00 GMT 2024

SECONDARY ACCESSION(S): PXD058251

REPOSITORIES: MassIVE

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Publications

Proteomic Profiling of Extracellular Vesicles Distinguishes Prostate Cancer Molecular Subtypes.

Ludwig Megan L ML Arafa Ali T AT Vinoo Saasha S Jones Jason C JC Day Abderrahman A Bergom Hannah E HE Sychev Zoi Z Horrmann Alec A Levinson Nicholas M NM Dehm Scott M SM Antonarakis Emmanuel S ES Hwang Justin J Drake Justin M JM

Journal of extracellular vesicles 20251001 10

Prostate cancer is the most common non-cutaneous cancer among men in the United States. Most prostate cancers are driven by androgen receptor (AR) signalling, but there are an increasing number of cases that lose AR and gain neuroendocrine (NE) features (AR-/NE+) or lack both (AR-/NE-). These latter subtypes are particularly aggressive and lethal. Extracellular vesicles (EVs) have shown great potential as biomarkers for non-invasive liquid biopsy assays, as EVs contain biomolecules from their ce ...[more]

PMID: 41063441

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Effects of prostate cancer patient urinary extracellular vesicles and hypoxic extracellular vesicles from prostate cancer cell lines on the transcriptomes of cancer-associated and normal fibroblasts

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2023-04-01 | E-MTAB-11317 | biostudies-arrayexpress

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RNA-seq of prostate cancer cell lines co-culture with osteoblasts or osteoclasts

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Effects of urinary extracellular vesicles from prostate cancer patients on the transcriptomes of cancer-associated and normal fibroblasts

Project description:Patient-derived prostate fibroblast primary cultures PCF-54 and PCF-55 were established from two specimens of PC tissues. EVs were isolated from urine samples of 3 patients with PC and 2 healthy males and used for the treatment of prostate fibroblast primary cultures and normal foreskin fibroblasts. The EV-treated fibroblasts were subjected to RNA sequencing analysis.

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Transcriptome profiling of Olaparib-resistant (OlapR) prostate cancer cell lines

Project description:Acquisition of resistance to the PARP inhibitor, Olaparib, constitutes a major challenge for the treatment of advanced prostate cancer. The purpose of this study was to identify molecular targets responsible for the development of acquired Olaparib resistance in advanced prostate cancer. Towards this goal, next-generation sequencing (NGS)-based gene expression profiling (RNA-Sequencing; RNA-Seq) was performed on castration-sensitive prostate cancer (CSPC)/Olaparib-sensitive LNCaP cells, castration-sensitive prostate cancer (CSPC)/Olaparib-resistant LN-OlapR cells, castration-resistant prostate cancer (CSPC)/Olaparib-sensitive C4-2B cells, and castration-resistant prostate cancer (CSPC)/Olaparib-resistant 2B-OlapR cells.

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Expression data from prostate cancer cell lines C4-2B (enzalutamide sensitive) and C4-2B-MDVR (enzalutamide resistant) cells

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Expression data from prostate cancer cell lines C4-2B (enzalutamide sensitive) and C4-2B-MDVR (enzalutamide resistant) cells

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: Proteome of Prostate Cell Line-derived Extracellular Vesicles

Project description:Project Description: Extracellular vesicles were isolated from conditioned media of prostate cancer cell lines PC-3M and LNCaP. Proteomic profiling was conducted on EVs to identify differential protein cargoes.

2020-06-27 | PXD019441 | Pride

Gene expression in human prostate cancer cell lines regulated by HIC1 gene

Project description:This study aimed to further our understanding of the role that hypermethylatioted in cancer 1 (HIC1) plays in prostate cancer (PCa) development. Microarrays were searched for some genes that had correlated expression with HIC1 mRNA. Our data showed that HIC1 promoter hypermethylation was presented in cell lines, tissues and plasma of PCa patients. According to fold-change screening between restoring expression of HIC1 and its respective control cells, both up-regulated and down-regulated genes were commonly observed in PC3 and C4-2B cells. The restoring expression HIC1 in PCa lines were respectively noted as PC3-HIC1 and C4-2B-HIC1 cells, and the respective controls were noted as C4-2B-GFP and PC3-GFP cells.

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