Dataset Information

Extracellular vesicles of a phytobeneficial bacterium trigger distinct systemic response in plant

ABSTRACT: Bacterial extracellular vesicles (EVs) are lipidic shuttles that play roles in virulence, inter-species competition, and in the induction of the host immune response. While they have primarily been investigated in animal-bacteria interactions, knowledge regarding phytobacterial EVs remains limited. Recent findings revealed that various biotic factors like hydroxycinnamic acids can regulate EVs production. Hydroxycinnamic acids, such as ferulic acid, are lignin components abundantly released in the plant environment, where they impact the ecology of numerous phytobacteria. Azospirillum sp. B510, a phytobeneficial bacterium, induces the accumulation of hydroxycinnamic acid derivatives in the plant and can metabolize them as carbon sources. We hypothesized that the presence of ferulic acid in the environment of Azospirillum sp. B510 would influence its EVs production in terms of size, quantity, and cargo. Conversely, we also proposed that EVs from this phytobacterium would influence plant metabolites and defense gene expression. Our results show both that ferulic acid (mimicking the plant environment) influences the content of EVs released by Azospirillum sp. B510 and that bacterial EVs also impact plant physiology at a systemic level according to their cargoes. This research provides the first evidence of a global effect of bacterial EVs on the plant and highlights the dynamics of plant-bacteria interactions mediated by EVs.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Azospirillum Sp. B510 (ncbitaxon:137722)

SUBMITTER: Ludovic Vial

PROVIDER: MSV000096701 | MassIVE | Thu Dec 19 03:53:00 GMT 2024

REPOSITORIES: MassIVE

ACCESS DATA

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Project description:The presence of anti-microbial phenolic compounds, such as the model compound ferulic acid, in biomass hydrolysates poses significant challenges to the widespread use of biomass in conjunction with whole cell biocatalysis or fermentation. Biofuel toxicity must also be overcome to allow for efficient production of next generation biofuels such as butanol, isopropanol, and others for widespread usage. Currently, these inhibitory compounds must be removed through additional downstream processing or sufficiently diluted to create environments suitable for most industrially important microbial strains. This study explores the high ferulic acid and n-butanol tolerance in Lactobacillus brevis (L. brevis), a lactic acid bacteria often found in fermentation processes, by global transcriptional response analysis. The transcriptional profile of L. brevis under ferulic acid and butanol stress reveals that the presence of ferulic acid primarily triggers the expression of membrane proteins to counteract ferulic acid induced changes in membrane fluidity and ion leakage. In contrast to the ferulic acid stress response, butanol addition to growing cultures uniquely induced the entire fatty acid synthesis pathway in the midst of a generalized stress response. Overexpression of the rate-limiting acetyl-CoA carboxylase subunits (AccABCD) in E. coli to increase lipid synthesis had no effect on butanol tolerance, suggesting that additional engineering is necessary to produce sufficient levels of appropriate fatty acids to confer butanol tolerance. Several promising routes for understanding both phenolic acid and butanol tolerance have been identified based upon these findings. These insights may be used to guide further engineering of model industrial organisms to better tolerate both classes of inhibitors in processed biomass used for biofuel production. Cultures were grown to OD ~ 0.2 in MRS media (baffled flasks), T = 30 C, 100 rpm. Butanol was then added to the cultures. Samples were harvested 15, 75, and 135 min after butanol addition. Each time point has 3 biological replicates, and dye swaps were incorporated into the microarray experiments.

Dataset Information

Extracellular vesicles of a phytobeneficial bacterium trigger distinct systemic response in plant

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets