Dataset Information

Cyanobacterial circadian regulation enhances bioproduction under subjective nighttime through rewiring of carbon partitioning dynamics, redox balance orchestration, and cell cycle modulation

ABSTRACT: GC-MS extracellular metabolite profiling of circadian clock regulation impacts of carbon partitioning between storage, growth, and product synthesis in Synechococcus elongatus PCC 7942 for enhanced bioproduction strategy investigation. Metabolomic targeted analysis of PCC 7942 light-dark cycle cultures transitioned to constant light revealed distinct temporal patterns in sucrose production. Culture samples (8 mL) were collected at 0, 0.5, 1, 2, 4, 6, and 8 hours then dried under speed vacuum and dissolved in pyridine and chemically derivatized by methoxyamination and trimethylsilylation for extracellular sucrose analysis. Data was analyzed using Agilent Mass Hunter. Culture samples (8 mL) were collected at 0, 0.5, 1, 2, 4, 6, and 8 hours then dried under speed vacuum and dissolved in pyridine and chemically derivatized by methoxyamination and trimethylsilylation for extracellular sucrose analysis.

INSTRUMENT(S): 8890 GC/MS

ORGANISM(S): Synechococcus Elongatus Pcc 7942 (ncbitaxon:1140)

SUBMITTER: Pavlo Bohutskyi