Proteomics

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Cyanobacterial circadian regulation enhances bioproduction under subjective nighttime through rewiring of carbon partitioning dynamics, redox balance orchestration, and cell cycle modulation


ABSTRACT: GC-MS extracellular metabolite profiling of circadian clock regulation impacts of carbon partitioning between storage, growth, and product synthesis in Synechococcus elongatus PCC 7942 for enhanced bioproduction strategy investigation. Metabolomic targeted analysis of PCC 7942 light-dark cycle cultures transitioned to constant light revealed distinct temporal patterns in sucrose production. Culture samples (8 mL) were collected at 0, 0.5, 1, 2, 4, 6, and 8 hours then dried under speed vacuum and dissolved in pyridine and chemically derivatized by methoxyamination and trimethylsilylation for extracellular sucrose analysis. Data was analyzed using Agilent Mass Hunter. Culture samples (8 mL) were collected at 0, 0.5, 1, 2, 4, 6, and 8 hours then dried under speed vacuum and dissolved in pyridine and chemically derivatized by methoxyamination and trimethylsilylation for extracellular sucrose analysis.

INSTRUMENT(S): 8890 GC/MS

ORGANISM(S): Synechococcus Elongatus Pcc 7942 (ncbitaxon:1140)

SUBMITTER: Pavlo Bohutskyi  

PROVIDER: MSV000096722 | MassIVE | Sat Dec 21 20:48:00 GMT 2024

REPOSITORIES: MassIVE

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Cyanobacterial circadian regulation enhances bioproduction under subjective nighttime through rewiring of carbon partitioning dynamics, redox balance orchestration, and cell cycle modulation.

Gilliam Ashley A   Sadler Natalie C NC   Li Xiaolu X   Garcia Marci M   Johnson Zachary Z   Veličković Marija M   Kim Young-Mo YM   Feng Song S   Qian Wei-Jun WJ   Cheung Margaret S MS   Bohutskyi Pavlo P  

Microbial cell factories 20250308 1


<h4>Background</h4>The industrial feasibility of photosynthetic bioproduction using cyanobacterial platforms remains challenging due to insufficient yields, particularly due to competition between product formation and cellular carbon demands across different temporal phases of growth. This study investigates how circadian clock regulation impacts carbon partitioning between storage, growth, and product synthesis in Synechococcus elongatus PCC 7942, and provides insights that suggest potential s  ...[more]

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