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Sucrose utilisation strategies in phototrophic purple bacteria: paving the way to sugar-rich by-product valorisation

ABSTRACT: The circular bioeconomy has become a crucial strategy for sustainable development, especially by upcycling organic-rich by-products from various industrial processes. Purple non-sulphur bacteria (PNSB) have emerged as excellent candidates in this field, demonstrating exceptional metabolic versatility. While the growth of PNSB on sugar-rich streams has been extensively explored, the sugar assimilation metabolism remains poorly understood. Here, we explore the metabolic mechanisms of sucrose utilisation in two phototrophic purple non-sulphur bacteria (PNSB), Rhodospirillum rubrum and Rhodobacter capsulatus. Our findings demonstrate distinct carbohydrate hydrolysis and assimilation capacities, as well as the use of different redox strategies for each species. Moreover, Rhodospirillum rubrum could only grow on sucrose when co-cultivated with Rhodobacter capsulatus. This trophic link between Rhodospirillum rubrum and Rhodobacter capsulatus in sucrose containing co-culture was characterised and resulted in significantly enhanced productivity compared to pure cultures. Finally, we demonstrate that the synergy observed between Rhodospirillum rubrum and Rhodobacter capsulatus can be successfully scaled up in a photobioreactor system. Our study highlights how fundamental knowledge of the metabolism, trophic link and general microbial ecology concept might be useful for the development of biobased resource recovery strategies.

INSTRUMENT(S): TripleTOF 6600

ORGANISM(S): Rhodospirillum Rubrum Atcc 11170 (ncbitaxon:269796)

SUBMITTER: Manon Gilson

PROVIDER: MSV000096927 | MassIVE | Fri Jan 24 04:57:00 GMT 2025

REPOSITORIES: MassIVE

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Project description:Sugarcane accumulates high concentrations of sucrose in the mature stalk, with numerous physiological processes in maturing stalk tissue both directly and indirectly involved. A considerable portion of carbohydrate metabolism is also devoted to cell wall synthesis and fibre production. Previously, we have identified differentially expressed transcripts correlated with sucrose accumulation and fibre production in various internodes of the sugarcane stalk. In this study, we have examined tissue-specific expression patterns to further explore gene pathways responsible for sucrose accumulation and fibre synthesis. We performed large-scale expression profiling of storage parenchyma, vascular bundles and rind dissected from a maturing stalk internode from field-grown commercial sugarcane harvested at 11 months of age. We identified 10 cellulose synthase subunit genes in sugarcane and examined significant differences in the expression of their corresponding transcripts and those of several sugar transporters between the different tissues. These were further correlated with differential expression patterns for transcripts of specific COBRA-like proteins and other proteins with acknowledged roles in cell wall metabolism. We found that the sugar transporters ShPST2a, ShPST2b and ShSUT4 were significantly up-regulated in storage parenchyma while ShSUT1 was up-regulated in vascular bundles. Two co-ordinately expressed groups of cell wall related transcripts were also identified. One group which is associated with primary cell wall synthesis (ShCesA1, ShCesA7, ShCesA9 and Shbk2l3) was up-regulated in parenchyma. The other group which is associated with secondary cell wall synthesis (ShCesA10, ShCesA11, ShCesA12 and Shbk-2) was up-regulated in rind. We also report an unexpected paucity in differential expression of cell wall-related genes in vascular bundles. Our results indicate that there is spatial separation for elevated expression of these important targets in both sucrose accumulation and cell wall synthesis, allowing for increased clarity in our understanding of sucrose transport and fibre synthesis in sugarcane.

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Sucrose utilisation strategies in phototrophic purple bacteria: paving the way to sugar-rich by-product valorisation

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