Project description:TMT analysis of proteomic changes in the gastrocnemius skeletal muscles of WT and Bmal1-KO mice, and Bmal1-KO mice rescued with AAV-mediated muscle-specific expression of Bmal1.
Project description:To understand the role of LSD1 in transcriptional regulation in muscle under glucocorticoid stress, RNA-seq analyses of gastrocnemius and soleus muscles of skeletal muscle-specific LSD1 KO mice (LSD1-mKO mice) and WT mice after dexamethasone were carried out. We found that LSD1 inhibition led to increased expression of muscle atrophy associated genes and slow fiber genes in gastrocnemius muscle but not in soleus muscle.
Project description:Transcriptomic profiles of gastrocnemius muscles from 18 weeks old, male, hind muscles unloaded mice treated with vehicle or4-phenylbutyrate (4-PBA)
Project description:In the context of developing an integrative proteogenomics strategy for assessing both differential gene expression and alternative splicing, we generated RNA-seq data (paired-end, 76 bp, 71 million reads per sample on average) from gastrocnemius muscles (right and left hind limb) from five wild-type mice and five HSALR mice (male, 10-12 weeks old) modelling the human disease Myotonic Dystrophy type 1 (DM1).
Project description:We immunoprecipitated GFP tagged ribosomes from Drd1 receptor-expressing cells in the striatum from adult WT and fragile X syndrome model mice and sequenced the co-purifying mRNA (IP). We also sequenced the RNA in the striatal lysate (total).
Project description:Genome-wide H3S10ph marks from mouse gastrocnemius muscles after 50 eccentric contractions compared to contralateral unstimulated gastrocnemius muscles from the same mice.
Project description:To determine if the absence of FKBP12 in cardiac tissue alters JPH2 and RYR2 interactions with other dyadic proteins, we immunoprecipitated JPH2 from hearts of FL, MCK-FKD, MHC-Cre+ only, and MHC-FKD mice.
Project description:We want to find differential expressed genes of gastrocnemius from transgenic mice of knockout DGAT1 and WT mice. We used microarrays to detail the global programme of differential expressed genes of gastrocnemius from transgenic mice of konckout DGAT1 and WT mice.
Project description:Study muscle regeneration in Emerin and Lamin AC KO mice. Gastrocnemius muscles were injected with cardiotoxin. Muscles were isolated at different time points. Keywords: Time-Series The specific aim is to generate staged muscle degeneration/regeneration of Emerin deficient and Lamin A/C deficient mice. Muscles will then be express profiled using Affymetrix arrays.