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Lipidome data of two wildtype pure cultures and two mutant strains of archaea

ABSTRACT: The lipidome of two wildtype pure cultures and two mutant strains of archaea were examined in this study, which included Nitrosopumilus maritimus strain SCM1, a Haloferax larsenii (JCM 13917), a Methanococcus maripaludis mutant strain (pMEV4-ma_1486) and a mutant strain of Sulfolobus acidocaldarius (p1561-grsB-grsA). The lipids were analyzed using a Waters SYNAPT G2-Si quadrupole time-of-flight mass spectrometer (qTOF) coupled to an electrospray ionization (ESI) source operated at positive ion mode.

INSTRUMENT(S): Waters SYNAPT G2-Si

ORGANISM(S): Archaea (ncbitaxon:2157)

SUBMITTER: Fengfeng Zheng Chuanlun Zhang

PROVIDER: MSV000097125 | MassIVE | Fri Feb 14 19:18:00 GMT 2025

REPOSITORIES: MassIVE

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Project description:Within the gut microbiome, Methanobrevibacter and Methanosphaera species are the prevailing methanogenic archaea. In general, these archaeal species interact widely with other members of the gut microbiome, subsequently facilitating the processes of digestion and fermentation within humans, thereby playing a significant role in the gut. Despite their significance, detailed characteristics and microbiome-host interactions remain largely unexplored. One potential mechanism for microbiome-host interaction and communication involves extracellular vesicles, which play a crucial role in both inter- and intra-kingdom interactions as well as intercellular communication. The production of extracellular vesicles has been confirmed for representatives of all three domains of life, eukaryotes, bacteria, and archaea. In this study, we report for the first time that human gut-derived archaea are capable of producing extracellular vesicles. Here, we present the ultrastructure, composition, proteome, and metabolome of these newly discovered archaeal extracellular vesicles (AEV) of M. smithii (strains ALI and GRAZ-2), Candidatus M. intestini, and Methanosphaera stadtmanae. Here, we describe their morphology, contents of archaeal extracellular vesicles (AEV) produced by the major methanogenic archaea of the human gut, namely Methanobrevibacter smithii (strains ALI and GRAZ-2), Candidatus M. intestini, and Methanosphaera stadtmanae. We also describe their interaction with human cell lines and ability to trigger immune responses. The findings show a high similarity of AEVs to their bacterial counterparts in size, morphology, and composition. Proteome and metabolome analysis demonstrate high similarities between vesicles derived from Methanobrevibacter species and are highly enriched in adhesin or adhesin-like proteins, suggesting an important role for archaeal-bacterial and archaeal-host interactions. Unless the specific role of AEVs could not be identified, their production itself suggests an intricate network of interdomain interactions shaping the dynamics of the human microbiome.

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Lipidome data of two wildtype pure cultures and two mutant strains of archaea

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